Showing 1–6 of 11 results

  • Bridge-It® Cyclic AMP (cAMP) all in one Assay Kit, 384-well format

    Bridge-It® Cyclic AMP (cAMP) all in one Assay Kit, 384-well format

    The Bridge-It® cAMP assay is highly specific assay to quantify cAMP in stimulated cells. ATP, AMP, and cGMP have all been tested for selectivity using the cAMP assay. No response was detected using the Bridge-It® cAMP assay with any of these compounds within the concentration range that is expected in biological samples (i.e., millimolar range for AMP and ATP, and micromolar range for cGMP).

    An all-in-one solution is added to activated cells and incubated for 30 minutes.  Clarified lysate samples are transferred to a black 384-well microplate to read the fluorescence signal.

  • Bridge-It® Cyclic AMP (cAMP) Designer Assay Kit, 96-well format

    Bridge-It® Cyclic AMP (cAMP) Designer Assay Kit, 96-well format

    The Bridge-It® cAMP assay is a highly specific assay to quantify cAMP in stimulated cells. ATP, AMP, and cGMP have all been tested for selectivity using the cAMP assay. No response was detected using the Bridge-It® cAMP assay with any of these compounds within the concentration range that is expected in biological samples (i.e., millimolar range for AMP and ATP, and micromolar range for cGMP).

    An all-in-one solution is added to activated cells and incubated for 30 minutes.  Clarified lysate samples are transferred to a black 96-well microplate to read the fluorescence signal.

  • Bridge-It® L-Tryptophan Fluorescence Assay Kit for Plasma and Serum Samples, 384-well format

    Bridge-It® L-Tryptophan Fluorescence Assay Kit for Plasma and Serum Samples, 384-well format

    The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.

    The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.

  • Bridge-It® L-Tryptophan Fluorescence Assay Kit, 384-well format

    Bridge-It® L-Tryptophan Fluorescence Assay Kit, 384-well format

    The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.

    The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.

  • Bridge-It® L-Tryptophan Fluorescence Assay Kit, 96-well format

    Bridge-It® L-Tryptophan Fluorescence Assay Kit, 96-well format

    The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.

    The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.

  • Bridge-It® S-Adenosyl Methionine (SAM) Fluorescence Assay Kit, 384-well format

    Bridge-It® S-Adenosyl Methionine (SAM) Fluorescence Assay Kit, 384-well format

    S-adenosyl methionine (also referred to as SAM, SAMe or AdoMet) plays a crucial role in the biological process of methylation in all types of organisms. In the methylation cycle, SAM serves as the donor of the methyl group used in the covalent modification of DNA and proteins.

     

    The Bridge-It® SAM fluorescence assay method exhibits highly desirable performance characteristics including a high (>5:1) signal to background (S/B) ratio, a broad linear dynamic range (0.5 µM – 20 µM), and, a detection sensitivity of 0.5 µM. This detection level (0.5 µM) is useful for quantifying SAM in most test samples of interest including biological fluids, cell culture and fermentation medium, and extracts of tissues and cells.