Patented Bridge-It® Assay Platform

Bridge-It® Assays are optimized to measure DNA binding proteins, their ligands, and co-regulators.

Mediomics’ Bridge-It® assay was based on a simple idea of splitting the DNA binding site for a protein into two DNA half-sites. The presence of the DNA-binding protein or one of its ligands can be detected by FRET (fluorescence resonance energy transfer).  [Learn More]

Features of the Bridge-It^® Assay System:

• Simple mix-and-measure system. Requires no technical manipulations other than mixing the test sample with the reaction mixture. Direct testing of samples without a dilution step. One step to mix test sample or standard with the reaction mixture.

• Fast. Read fluorescent signal after 30 minutes of incubation

• Quantitative.

• Reproducible. Minimal assay variability. It does not require antibodies or radioactive probes, which are more variable from lot-to-lot.

• Compatible. Assay is compatible with most fluorescence plate readers and optical multi-well plates. 

• High-throughput screening capacity.

• Expandable portfolio. Platform technology can be expanded to include additional DNA-binding proteins and co-regulators or ligands. Combination kits to measure the activity of multiple DNA binding proteins.

Patented FRET-PINCER® Assay Platform

FRET-PINCER® Assays are a unique system to quantify the concentration of biomarkers, cells, and pathogens in a broad range of biological samples.

The patent-protected FRET-PINCER® Assay system detects the concentration of the target molecule by bringing together complementary single-stranded DNAs and their attached fluorophores to produce fluorescent light through FRET (fluorescence resonance energy transfer).

Mediomics’ PINCER® assay uses antibody binding specificity combined with DNA base-pair recognition to create a simple and reproducible assay system. [Learn More]

Features of the PINCER® Assay System:

• Easy. Mix test sample or standard with the Assay solution and incubate at room temperature

• Fast. Read fluorescent signal after 30 minutes of incubation

• Broad Detection Range. Direct testing of samples without a dilution step

• Flexible. Assay is compatible with most fluorescence plate readers

• High-Throughput Screening Compatible.

Patented TRF-PINCER® Assay Platform

TRF-PINCER® Assays are a unique system to quantify the concentration of biomarkers, cells, and pathogens in a broad range of biological samples.

The patent-protected TRF-PINCER® Assay platform system detects the concentration of the target molecule by bringing together complementary single-stranded DNAs and their attached fluorophores to produce fluorescent light with Time-Resolved Fluorometry (TRF) sensitivity.

Mediomics’ PINCER® assay uses antibody binding specificity combined with DNA base-pair recognition to create a simple and reproducible assay system. TRF-PINCER is an extension of the FRET-PINCER system with an extended detection range capability. [Learn More]

Features of the PINCER® Assay System:

• Easy. Mix test sample or standard with the Assay solution and incubate at room temperature

• Fast. Read fluorescent signal after 30 minutes of incubation

• Broad Detection Range. Direct testing of samples without a dilution step

• Flexible. Assay is compatible with most fluorescence plate readers

• High-Throughput Screening Compatible.