Need Help? Bulk Orders? Ordering by P.O. number? Contact Customer Service for further assistance. Phone: 1-314-971-3028 | Email: [email protected]

  • No products in the cart.

Customer Publications

79. Suganuma T, et al. MPTAC Determines APP Fragmentation via Sensing Sulfur Amino Acid Catabolism. Cell reports. 24.6 (2018): 1585-1596.

--“SAMe Quantification: 8.6–11.1 μg cytoplasmic extracts and 5.7 μg nuclear extracts were subjected to the Bridge-It SAM Fluorescence Assay, following the manufacturer’s instructions (1-1-1004B; Mediomics).”

78. Lane DRJ, et al. Coupling of the polyamine and iron metabolism pathways in the regulation of proliferation: Mechanistic links to alterations in key polyamine biosynthetic and catabolic enzymes. Biochim Biophys Acta. 2018 May 16.

--“The determination of AdoMet levels were assessed using the Bridge-It® SAM Fluorescence Assay (Mediomics LLC, St. Louis, MO), based on the manufacturer's instructions.”

77. Huimin N, Ponomarova O, et al. C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development. Cell Reports. 2018 March 20.

--“SAM was measured using the Bridge-It S-Adenosyl Methionine Fluorescence Assay Kit purchased from Mediomics following the manufacturer’s recommendations.”

76. Riopelet M, Seo JB, et al. Chronic fractalkine administration improves glucose tolerance and pancreatic endocrine function. The Journal of Clinical Investigation. March 2018.

--“Intracellular cAMP, ADP, and ATP levels in αTC1 or Min6 cells were measured using a Bridge-It cAMP assay kit (Mediomics LLC)… Bridge-It Cyclic AMP Designer Assay was used to determine intracellular cAMP levels per the manufacturer’s instructions (catalog 122934; Mediomics LLC).”

75. Chang CK, et al. Branched-chain amino acids and arginine improve physical but not skill performance in two consecutive days of exercise. Journal of Sports Science and Medicine. December 2017.

74. Zhou Z, et al. Physiological and molecular mechanisms associated with performance, immunometabolic status, and liver function in transition dairy cows fed rumen-protected methionine or choline. Journal of Animal Science. 11 April 2016.

-- “Liver tissue L-methionine was measured at -10, 7, 20, and 30 d relative to parturition using a commercial kit (Cat. No. TRF-1-1-1006B; Mediomics, LLC; St. Louis, MO)…”

73. Jung J, Kim L, et al. “Nicotinamide metabolism regulates glioblastoma stem cell maintenance.” JCI Insight. 2017 May; 2(10): e90019.

--"For measurement of L-methionine, homocysteine, SAM, SAH, and NAD+ conventional assay kits were used for their detection as follows: Bridge-It L-methionine fluorescence assay kit (Mediomics)…”

72. Mahmouda ME, Fereiga R, Nishikawaa Y. Involvement of host defense mechanisms against Toxoplasma gondii infection in anhedonic and despair-like behaviors in mice. American Society for Microbiology. 2017 January 30.

-- “Plasma L-Trp was assayed 239 with a Bridge-It L-Trp Fluorescence Assay (Mediomics, St. Louis, MO)”

71. Portugal L, et al. Toxicity of Cry1A toxins from Bacillus thuringiensis to CF1 cells does not involve activation of adenylate cyclase/PKA signaling pathway. Insect Biochemistry and Molecular Biology. 16 November 2016.

-- “The cAMP levels were determined by using the Bridge-It cAMP designer fluorescence assay system (Mediomics, LLC, St. Louis), according to the manufacturer's specifications.”

70. Ostergaard O, et al. Quantitative protein profiling of Chlamydia trachomatis growth forms reveals defense strategies against tryptophan starvation. Molecular and Cellular Proteomics. 2016 October 26.

-- “After sterile filtration, the Trp content in the spent culture medium was determined by the Bridge-It® L-Trp assay according to the manufacturer’s instructions (Mediomics, LLC, St. Louis, MO).”

69. Chang CK, et al. Branched-chain amino acids, arginine, citrulline alleviate central fatigue after 3 simulated matches in taekwondo athletes: a randomized controlled trial. Journal of Sports Science and Medicine. 2016 July 13.

--“Plasma free tryptophan concentration was analyzed with a fluorescence assay (Bridge-It, Mediomics, St. Louis, MO, USA)."

68. Chang CK, et al. Prevention of perceptual-motor decline by branched-chain amino acids, arginine, citrulline after tennis match. Scandinavian Journal of Medicine and Science in Sports. 2016 July 1.

--"Plasma total tryptophan concentration was analyzed by a fluorescence assay (Bridge-It, Mediomics, St. Louis, Missouri, USA)."

67. Blain GM, Mangum TS, Sidhu SK, Weavil JC, Hureau TJ, Jessop JE, Bledsoe AD, Richardson RS, Amann M. Group III/IV muscle afferents limit the intramuscular metabolic perturbation during whole body exercise in humans. J Physiol. 2016 May 31.

--“Tryptophan levels were analyzed using the Bridge-It® L-Tryptophan Fluorescence Assay (Mediomics, LLC, St. Louis, MO, USA).”

66. Kolar K, Wischhusen HM, Muller K, Kalrsson M, Weber W, Zubriggen M. A synthetic mammalian network to compute population borders based on engineered reciprocal cell-cell communication. BioMed Central: Systems Biology. 2015 December 30.

-- "Interleukin-4 (IL-4) and L-tryptophan were quantified in the culture medium using an IL-4 ELISA kit (Pepro Tech, cat. no. 900-K14) or the Bridge-IT L-Tryptophan Fluorescence Assay (Mediomics, cat. no. 1-1-1002A) according to the manufacturer’s instructions, respectively."

65. Mahmoud, Motamed Elsayed, et al. Induction of depression-related behaviors by reactivation of chronic Toxoplasma gondii infection in mice. Behavioural Brain Research. 2015 November 7.

--“Plasma l-Trp was measured by fluorescence (excitation: 485 nm, emission: 665 nm) using the Bridge-It l-Trp Fluorescence Assay kit (Mediomics, LLC).”

64. Murray TVA, et al. gavageNADPH oxidase 4 regulates homocysteine metabolism and protects against acetaminophen-induced liver damage in mice. Free Radical Biology and Medicine. 2015 October 22.

--“Methionine was measured in extracts from Wt and Nox4 -/- mouse livers using a Bridge-It® L-Methionine (L-Met) Fluorescence Assay (Mediomics) as per the manufacturer's protocol.”

63. Koczor CA, et al. AZT-induced Mitochondrial Toxicity: An Epigenetic Paradigm for Dysregulation of Gene Expression through Mitochondrial Oxidative Stress. Physiological Genomics. 2015 July 21.

--“For S-adenosylmethionine (SAM) 142 quantitation, a SAM fluorescence assay was utilized (Mediomics, St. Louis, MO).

62. Oh SK, et al. Pannexin 3 is required for normal progression of skeletal development in vertebrates. The FASEB Journal. 2015 July 16.

--“Measurement of intracellular cAMP After 4 d of micromass culture, the cAMP levels in the cells were measured using the Bridge-It cAMP Designer fluorescence assay kit (Mediomics, St. Louis, MO, USA).”

61. McNelis JC, et al. GPR43 potentiates beta cell function in obesity. Diabetes Journal. 2015 May 28.

--“Intracellular cAMP analysis: Intracellular cAMP levels in Min6 cells and isolated islets were measured using Bridge-It cAMP designer fluorescence Assay Kit (Mediomics).”

60. Dann SG, et al. Reciprocal regulation of amino acid import and epigenetic state through Lat1 and EZH2. The EMBO Journal. 2015 May 15.

59. Röhrig UF, Majjigapu SR, Vogel P, Zoete V, Michielin O. Challenges in the Discovery of Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors. J. Med. Chem. 2015 May 13.

58. Chang CK, Chang Chien KM, Chang JH, Huang MH, Liang YC, Liu TH. Branched-Chain Amino Acids and Arginine Improve Performance in Two Consecutive Days of Simulated Handball Games in Male and Female Athletes: A Randomized Trial. PLOS One. 2015 March 24.

--“Plasma free tryptophan concentration was analyzed with a fluorescence assay according to manufacturer's recommendation (Bridge-It, Mediomics, St. Louis, MO, USA).”

57. Nishikawa K, et al. DNA methyltransferase 3a regulates osteoclast differentiation by coupling to an S-adenosylmethionine– producing metabolic pathway. Nature Medicine 2015 Feb 23; 21, 281–287.

-- “ATP and SAM in osteoclast precursors and osteoclasts were detected using the Kinshiro ATP Luminescence kit (CosmoBio) and Bridge-It SAM Fluorescence Assay Kit (Mediomics), respectively.”

56. Mews P, Zee BM, Liu S, Donahue G, Garcia BA, Berger SL. Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence. Mol Cell Biol. 2014 Nov;34(21):3968-80.

-- “To quantify SAM levels, the Bridge-It SAM fluorescence assay (Mediomics) was used according to the manufacturer's instructions.”

55. Piesiewicz A, Kedzierska U, Turkowska E, Adamska I, Majewski PM. Seasonal postembryonic maturation of the diurnal rhythm of serotonin in the chicken pineal gland. Chronobiol Int. 2014 Sep 15.

-- “...duplicate 10 µl aliquots were taken to measure TRP concentration with a Bridge-It L-tryptophan Fluorescence Assay Kit (Mediomics, St. Louis, MO).

54. Batra V, Verma P. Dietary L-methionine supplementation mitigates gamma-radiation induced global DNA hypomethylation: Enhanced metabolic flux towards S-adenosyl-l-methionine (SAM) biosynthesis increases genomic methylation potential. Food Chem Toxicol. 2014 Jul;69:46-54.

-- "...l-methionine and SAM assay kit was purchased from Mediomics Inc. (St. Louis, MO, USA)."

53. Urata Y, et al. IL-1β Increases Expression of Tryptophan 2,3-dioxygenase and Stimulates Tryptophan Catabolism in Endometrioma Stromal Cells. AJRI, 26 Jun 2014.

--"Concentration of tryptophan in conditioned media was measured using Bridge-It L-Tryptophan Fluorescence Assay (Mediomics, St. Louis, MO, USA) according to the manufacturer's protocol."

52. Salvatori AS, Elrick MM, Samson WK, Corbett JA, Yosten GL. Neuronostatin inhibits glucose-stimulated insulin secretion via direct action on the pancreatic α-cell. Am J Physiol Endocrinol Metab. 2014 Jun 1;306(11):E1257-63.

-- "Sample pellets were resuspended in appropriate assay buffers, and cAMP was measured by radioimmunoassay (PerkinElmer), BioTrak enzyme immunoassay (Amersham, Piscataway, NJ), or Bridge-It fluorometric assay (Mediomics, St. Louis, MO) according to the manufacturers' protocols."

51. Tan W, Thomas P. Activation of the Pi3k/Akt pathway and modulation of phosphodiesterase activity via membrane progestin receptor-alpha (mPRalpha) regulate progestin-initiated sperm hypermotility in Atlantic croaker. Biol Reprod. 2014 May;90(5):105

-- "Phosphodiesterase activity was measured using the Bridge-It cAMP PDE Assay kit (Mediomics, St. Louis, MO)."

50. Saalau-Bethell SM, Berdini V, Cleasby A, Congreve M, Coyle JE, Lock V, Murray CW, O'Brien MA, Rich SJ, Sambrook T, Vinkovic M, Yon JR, Jhoti H. Crystal structure of human soluble adenylate cyclase reveals a distinct, highly flexible allosteric bicarbonate binding pocket. ChemMedChem. 2014 Apr;9(4):823-32.

-- "The enzymatic activity of hsolAC was measured in 96-well (black) plate format using the Mediomics Bridge-It designer cAMP kit (cat #122 935, Mediomics, LLC, St. Louis, MO, USA)."

49. Zhu H, Guariglia S, Li W, Brancho D, Wang ZV, Scherer PE, Chow CW. Role of extracellular signal-regulated kinase 5 in adipocyte signaling. J Biol Chem. 2014 Feb 28;289(9):6311-22.

-- "Phosphodiesterase activity was determined by using the Bridge-It cAMP-PDE assay kits from Mediomics."

48. Ishikawa M1, Iwamoto T, Fukumoto S, Yamada Y. Pannexin 3 inhibits proliferation of osteoprogenitor cells by regulating Wnt and p21 signaling. J Biol Chem. 2014 Jan 31;289(5):2839-51.

-- "The level of cAMP was determined with a Bridge-It cAMP designer fluorescence assay kit (Mediomics)."

47. Mandal S, Mukhopadhyay S, Bandhopadhyay S, Sen G, Biswas T. 14-Deoxyandrographolide alleviates ethanol-induced hepatosteatosis through stimulation of AMP-activated protein kinase activity in rats. Alcohol. 2014 Mar;48(2):123-32

-- "Intracellular cAMP levels in rat hepatocytes and human hepatoma HepG2 cells (10 5 /mL) were measured by using Bridge-It ® cAMP Fluorescence Assay kits (Mediomics, LLC)."

46. Stollar V, Mensah V, Adams S, Li ML. Evolution of Sindbis virus with a low-methionine-resistant phenotype is dependent both on a pre-existing mutation and on the methionine concentration in the medium. PLoS One. 2013;8(3):e60504.

-- "The concentration of AdoMet in cells was determined by the Bridge-It S-Adenosyl Methionine Fluorescence Assay following the manufacturer's protocol (Mediomics)."

45. Lo TF, Tsai WC, Chen ST. MicroRNA-21-3p, a berberine-induced miRNA, directly down-regulates human methionine adenosyltransferases 2A and 2B and inhibits hepatoma cell growth. PLoS One. 2013 Sep 30;8(9):e75628.

-- "SAM levels in the supernatants were quantified using the Bridge-It SAM fluorescence assay kit (Mediomics) and detected using SpectraMax plate reader (Molecular Devices) according to the manufacturer's instructions."

44. Garciandia A, Suarez T. The NMRA/NMRAL1 homologue PadA modulates the expression of extracellular cAMP relay genes during aggregation in Dictyostelium discoideum. Dev Biol. 2013 Sep 15;381(2):411-22.

-- "Phosphodiesterase activity was measured in freshly obtained conditioned media, from 1×10 7 cells/mL starved for 6.5 h (plated at 2×10 6 cells/cm2), using Bridge-It cAMP_PDE Assay (Mediomics, LLC, IL, USA)."

43. Masyuk AI, et al. Ciliary subcellular localization of TGR5 determines the cholangiocyte functional response to bile acid signaling. Am J Physiol Gastrointest Liver Physiol. 2013 Jun 1;304(11):G1013-24.

--"Measurement of cAMP. cAMP levels in cholangiocytes were measured by the Bridge-It cAMP designer cAMP assay (Mediomics)."

42. Lee YS et al. The fractalkine/CX3CR1 system regulates β cell function and insulin secretion. Cell. 2013 Apr 11;153(2):413-25.

-- "Intracellular cAMP level of Min6 cells was measured using Bridge-it cAMP assay kit (Mediomics, USA)."

41. Nan YS, et al. Different effects of dibutyryl cAMP on monolayer permeability in human aortic and coronary arterial endothelial cells. African Journal of Microbiology Research Vol.6(5) 897-903, 2012

-- "Measurement of intracellular cAMP in endothelial cells Intracellular cAMP was measured using a Bridge-It cAMP designer fluorescence assay kit (Mediomics, St. Louis, MO, USA) according to the manufacturer's instructions."

40. Rascan et al. S-Adenosylmethionine: A Novel Factor in the Individualization of Thiopurine Therapy.

-- "The commercially available assay for SAM determination is a Mediomics Bridge-It® fluorescence assay based on a combination of fluorescence measurement techniques…"

39. Piesiewicz A, Kedzierska U, Adamska I, Usarek M, Zeman M, Skwarlo-Sonta K, Majewski PM. Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken. Gen Comp Endocrinol. 2012 Nov 1;179(2):143-51.

-- " Pineal glands were sonicated (Bandelin electronic) in 25 μl of ice-cold sodium phosphate buffer (50 mM, pH 6.8) and duplicate 10 μl aliquots were taken to measure TRP concentration using a Bridge-It l -tryptophan Fluorescence Assay Kit (Mediomics).

38. Schrötter A, Pfeiffer K, El Magraoui F, Platta HW, Erdmann R, Meyer HE, Egensperger R, Marcus K, Müller T. The amyloid precursor protein (APP) family members are key players in S-adenosylmethionine formation by MAT2A and modify BACE1 and PSEN1 gene expression-relevance for Alzheimer's disease. Mol Cell Proteomics. 2012 Nov;11(11):1274-88.

-- "S-adenosylmethionine (SAM) levels were measured with Bridge-It™ S-adenosylmethionine Fluorescence Assay (Mediomics, St. Louis, MO, USA) using a 384-well microplate format."

37. Bacchus W, Lang M, El-Baba MD, Weber W, Stelling J, Fussenegger M. Synthetic two-way communication between mammalian cells. Nat Biotechnol. 2012 Oct;30(10):991-6.

-- "L-tryptophan levels in the medium and serum were profiled using a fluorescence-based assay kit (Mediomics, St. Louis, MO, USA, cat. no. 1-1-1001B)."

36. Kanellopoulos AK, Semelidou O, Kotini AG, Anezaki M, Skoulakis EM. Learning and memory deficits consequent to reduction of the fragile X mental retardation protein result from metabotropic glutamate receptor-mediated inhibition of cAMP signaling in Drosophila. J Neurosci. 2012 Sep 19;32(38):13111-24.

-- "The Bridge-It cAMP designer fluorescence assay system (Mediomics) was used to measure cAMP levels in all samples."

35. Baldwin AC, Green CD, Olson LK, Moxley MA, Corbett JA. A role for aberrant protein palmitoylation in FFA-induced ER stress and β-cell death. Am J Physiol Endocrinol Metab. 2012 Jun 1;302(11):E1390-8.

-- "Insulin accumulating in the incubation buffer was measured using the Human Insulin Pincer assay kit (Mediomics, St. Louis, MO).

34. McGraw, DW. A Polymorphic Change in Amino Acid 220 (Ser220Cys) Of The Human ß2- Adrenergic Receptor Encodes Receptors With Different Gs-Coupled Signal Transduction Properties.

-- "Cellular production of 125 cAMP was measured using a fluorescent assay kit (Mediomics, MO)."

33. Meininger D, Zalameda L, Liu Y, Stepan LP, Borges L, McCarter JD, Sutherland CL. Purification and kinetic characterization of human indoleamine 2,3-dioxygenases 1 and 2 (IDO1 and IDO2) and discovery of selective IDO1 inhibitors. Biochim Biophys Acta. 2011 Dec;1814(12):1947-54.

-- "The activities and inhibition of IDO1, IDO2 and TDO were determined by measuring the production of the kynurenine product by kynurenine fluorescence or by the consumption of the Trp substrate using the Bridge-IT tryptophan fluorescence assay (Mediomics, St. Louis, MO)."

32. Abukhashim M, Wiebe GJ, Seubert JM. Regulation of forskolin-induced cAMP production by cytochrome P450 epoxygenase metabolites of arachidonic acid in HEK293 cells. Cell Biol Toxicol. 2011 Oct;27(5):321-32.

-- "Intracellular cAMP levels were measured by using commercially available kits (Mediomics)."

31. Kazutaka S, Winnall WR, Muir JA, Hedger MP. Regulation of Sertoli cell activin A and inhibin B by tumour necrosis factor α and interleukin 1α: interaction with follicle-stimulating hormone/adenosine 3',5'-cyclic phosphate signalling. Mol Cell Endocrinol. 2011 Mar 30;335(2):195-203.

-- "The commercially available assay for SAM determination is a Mediomics Bridge-It® fluorescence assay based on a combination of fluorescence measurement techniques."

30. Reszka KJ, Sallans L, Macha S, Brown K, McGraw DW, Kovacic MB, Britigan BE. Airway peroxidases catalyze nitration of the {beta}2-agonist salbutamol and decrease its pharmacological activity. J Pharmacol Exp Ther. 2011 Feb;336(2):440-9.

-- "The ability of native and nitrated salbutamol to stimulate cAMP production in airway smooth muscle cells was measured by a fluorescent detection assay using a commercially available kit (Mediomics, St. Louis, MO)."

29. Iwamoto T, Nakamura T, Doyle A, Ishikawa M, de Vega S, Fukumoto S, Yamada Y. Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation. J Biol Chem. 2010 Jun 11;285(24):18948-58.

-- "The level of cAMP was determined with a Bridge-It cAMP Designer fluorescence assay kit (Mediomics)."

28. Batra V, Sridhar S, Devasagayam TP. Enhanced one-carbon flux towards DNA methylation: Effect of dietary methyl supplements against γ-radiation-induced epigenetic modifications. Chem Biol Interact. 2010 Feb 12;183(3):425-33.

-- “…SAM assay kit was purchased from Mediomics Inc. (St. Louis, MO, USA)…”

27. Piesiewicz A, Podobas E, Kedzierska U, Joachimiak E, Markowska M, Majewski P, Skwarlo-Sonta K. Season-Related Differences in the Biosynthetic Activity of the Neonatal Chicken Pineal Gland.The Open Ornithology journal, 2010, 3, 134-140.

-- "L-tryptophan was measured in duplicate pineal sonicate samples using a Bridge-It L-Tryptophan Fluorescence Assay Kit (Mediomics), according to the manufacturer’s protocol."

26. Nifoussi SK. Posttranslational regulation of protein function and stability at the mitochondria and beyond. PhD (Doctor of Philosophy) thesis, University of Iowa, 2010.

"…Bridge-It cAMP designer assay (Mediomics) was used based on manufacturers protocol.”

25. Alvarado JJ, Nemkal A, Sauder JM, Russell M, Akiyoshi DE, Shi W, Almo SC, Weiss LM. Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470. Mol Biochem Parasitol. 2009 Dec;168(2):158-67.

-- "Recombinant human MetAP2 was purchased from Mediomics (St Louis, MO)."

24. Watson JA, Watson CJ, McCrohan AM, Woodfine K, Tosetto M, McDaid J, Gallagher E, Betts D, Baugh J, O'Sullivan J, Murrell A, Watson RW, McCann A. Generation of an epigenetic signature by chronic hypoxia in prostate cells. Hum Mol Genet. 2009 Oct 1;18(19):3594-604.

-- "SAM levels were quantified using the Bridge-It® SAM fluorescence assay (Mediomics)."

23. Steffens AA. Low-dose of sodium arsenite causes delayed differentiation in C2C12 mouse myoblast cells through the repression of the transcription factor myogenin. 2009. Thesis. Clemson University.

-- “S-adenosyl methionine (SAM) concentrations were determined using the Bridge-It SAM Fluorescence Assay (Mediomics, St. Louis, MO).”

22. Desaulniers D, Xiao GH, Lian H, Feng YL, Zhu J, Nakai J, Bowers WJ. Effects of mixtures of polychlorinated biphenyls, methylmercury, and organochlorine pesticides on hepatic DNA methylation in prepubertal female Sprague-Dawley rats. Int J Toxicol. 2009 Jul-Aug;28(4):294-307.

21. Reszka KJ, McGraw DW, Britigan BE. Peroxidative metabolism of beta2-agonists salbutamol and fenoterol and their analogues. Chem Res Toxicol. 2009 Jun;22(6):1137-50.

20. Akullian LC, Stevenson CA, Yurkovetskiy AV, Benson JD, Fram RJ, Lowinger, TB. Anti-angiogenic and anti-tumor activity of XMT-1107, a fumagillol-derived polymer conjugate, and its in vivo release product XMT-1191. Proc Am Assoc Cancer Res. 2009; abstract 670.

-- “MetAP2 inhibition assays were performed at Mediomics, St. Louis, MO. Special thanks to Yie-Hwa Chang.”

19. Furukawa F, Kanehara S, Harano F, Shinohara S, Kamimura J, Kawabata S, Igarashi S, Kawamura M, Yamamoto Y, Miyachi Y. Effects of adenosine 5'-monophosphate on epidermal turnover. Arch Dermatol Res. 2008 Oct;300(9):485-93.

-- "Intracellular cAMP level was measured with Bridge-It cAMP designer fluorescence assay kit (Mediomics, LLC, St. Louis, MO, USA)."

18. Anatoliy I. Masyuk, Sergio A. Gradilone, Jesus M. Banales, Bing Q. Huang, Tatyana V. Masyuk, Seung-Ok Lee, Patrick L. Splinter, Angela J. Stroope, and Nicholas F. LaRusso. Cholangiocyte primary cilia are chemosensory organelles that detect biliary nucleotides via P2Y12 purinergic receptors. Am J Physiol Gastrointest Liver Physiol. 2008 October; 295(4): G725–G734.

-- “cAMP levels were measured by the Bridge-It cAMP designer cAMP assay (Mediomics) and expressed in pmol of cAMP per well.”

17. Raake PW, Vinge LE, Gao E, Boucher M, Rengo G, Chen X, DeGeorge BR Jr, Matkovich S, Houser SR, Most P, Eckhart AD, Dorn GW 2nd, Koch WJ. G protein-coupled receptor kinase 2 ablation in cardiac myocytes before or after myocardial infarction prevents heart failure. Circ Res. 2008 Aug 15;103(4):413-22.

16. Coppin JF, Qu W, Waalkes MP. Interplay between Cellular Methyl Metabolism and Adaptive Efflux during Oncogenic Transformation from Chronic Arsenic Exposure in Human Cells. J Biol Chem. 2008 July 11; 283(28): 19342–19350.

-- “SAM levels were assayed according to manufacturer instructions using the Bridge-it® SAM fluorescence assay kit (Mediomics, St. Louis, MO).”

15. Chan A, Tchantchou F, Graves V, Rozen R, Shea TB. Dietary and genetic compromise in folate availability reduces acetylcholine, cognitive performance and increases aggression: critical role of S-adenosyl methionine. J Nutr Health Aging. 2008 Apr;12(4):252-61.

-- “SAM levels in homogenates of forebrain were determined by comparison to SAM standards using a Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit (Mediomics, LLC).”

14. Masyuk TV, Masyuk AI, Torres VE, Harris PC, Larusso NF. Octreotide inhibits hepatic cystogenesis in a rodent model of polycystic liver disease by reducing cholangiocyte adenosine 3',5'-cyclic monophosphate. Gastroenterology. 2007 Mar;132(3):1104-16.

-- “cAMP concentrations were determined by the Bridge-It cAMP designer cAMP assay (Mediomics, LLC, St Louis, MO).”

13. Kelley DJ, Davidson RJ, Elliott JL, Lahvis GP, Yin JCP, Bhattacharyya B.The Cyclic AMP Cascade Is Altered in the Fragile X Nervous System. PLoS ONE. 2007; 2(9): e931.

-- “The Bridge-It cAMP designer fluorescence assay system (Mediomics, LLC, St. Louis, MO) was used to measure cyclic AMP levels in all samples.”

12. Chan A, Shea TB. Supplementation with apple juice attenuates presenilin-1 overexpression during dietary and genetically-induced oxidative stress. J Alzheimers Dis. 2006 Dec;10(4):353-8.

-- “… SAM levels in homogenates of forebrain and in AJC were determined by comparison to SAM standards us- ing a Bridge-It S-Adenosyl Methionine (SAM) Fluo- rescence Assay Kit (Mediomics, LLC) assayed on a Hitachi F2000 fluorescent spectrophotometer.”

11. Anatoliy I. Masyuk, Tatyana V. Masyuk, Patrick L. Splinter, Bing Q. Huang, Angela J. Stroope, and Nicholas F. LaRusso (2007) Cholangiocyte Cilia Detect Changes in Luminal Fluid Flow and Transmit Them into Intracellular Ca2+ and cAMP Signaling. Gastroenterology. 2006 September; 131(3): 911–920.

-- “The concentrations of cAMP were determined by the Bridge-It™ cAMP designer cAMP assay (Mediomics, LLC, St. Louis, MO).”

10. Wilson BD, Ii M, Park KW, Suli A, Kock GAH, Sorensen LK, Suh W, Larrieu-Lahargue F, Urness LD, Thomas KR, Chien CB, Losordo DW, Li DY. Netrins Promote Developmental and Therapeutic Angiogenesis. Science. 2006 August 4; 313(5787): 640–644.

-- “Two different cAMP measurement systems were utilized (Mediomics and Assay Designs); similar results were obtained with each assessment.”

9. Hannig G, Lazarus DD, Bernier SG, Karp RM, Lorusso J, Qiu D, Labenski MT, Wakefield JD, Thompson CD, Westlin WF. Inhibition of melanoma tumor growth by a pharmacological inhibitor of MetAP-2, PPI-2458. Int J Oncol. 2006 Apr; 28(4):955-63.

-- “ For protein detection in cell lysates, we used the following antibodies: MetAP-1 polyclonal antibody (Mediomics)…”

8. Kalhan R, Thavarajah K, Nlend MC, Nair A, Smith LJ, Sporn PHS. The soy isoflavone genistein blocks transforming growth factor b1‐stimulated lung fibroblast to myofibroblast transformation. Journal of Investigative Medicine: 1 March 2006 - Volume 54 - Issue 2 - p S354.

-- “Using an assay in which reductions in fluores- cence intensity are associated with increasing cAMP levels (Mediomics, LLC), we quantified cAMP levels in neurospheres (NS) and differentiated cells (DC) from the human cortical fragile X (M049) and control…”

7. Brennan JP, Bardswell SC, Burgoyne JR, Fuller W, Schroder E, Wait R, Begum S, Kentish JC, Eaton P. (2006)Oxidant-induced Activation of Type I Protein Kinase A Is Mediated by RI Subunit Interprotein Disulfide Bond Formation. J. Biol. Chem. 281: 21827-21836.

-- “cAMP was measured in cells treated with H2O2 or isoprenaline using a Bridge-It cAMP designer fluorescence assay kit according to the manufacturer's guidelines (Mediomics).”

6. Zhang X, Candas M, Griko NB, Taussig R, Bulla LA Jr. (2006) A mechanism of cell death involving an adenylyl cyclase/PKA signaling pathway is induced by the Cry1Ab toxin of Bacillus thuringiensis. Proc Natl Acad Sci U S A.103:9897-9902.

-- “Involvement of AC/PKA pathway in Cry1Ab toxin-induced cytotoxicity. (A) Stimulation of cAMP production by Cry1Ab toxin. Cells were incubated with isobutyl-1-methylxanthine (0.5 mM) before the addition of Cry1Ab, and cAMP was measured by using the Bridge-It cAMP Assay (Mediomics) in S5 and H5 cells (3 x 104).”

5. White CA, Sowa JR, Blake AD. A fluorescence-based second messenger assay to identify small molecule antagonists of somatostatin. 2006, ASPET, 48 (1).

-- "Intracellular cAMP levels were quantified using a Bridge-It cAMP Designer Fluorescence Assay (Mediomics, LLC, St Louis, MO), which replaced more conventional radioimmunoassay methods."

4. Cooper AC, Karp RM, Clark EJ, Taghizadeh NR, Hoyt JG, Labenski MT, Murray MJ, Hannig G, Westlin WF, Thompson CD. (2006) A novel methionine aminopeptidase-2 inhibitor, PPI-2458, inhibits non-Hodgkin's lymphoma cell proliferation in vitro and in vivo. Clin Cancer Res. 12:2583-2590.

-- “Human recombinant MetAP-2 (Mediomics, St. Louis, MO), prebound to the biotinylated PPI-2458 analogue, was used to generate a standard curve for quantitation for each individual assay.”

3. Keys JR, Zhou RH, Harris DM, Druckman CA, Eckhart AD. (2005) Vascular smooth muscle overexpression of G protein-coupled receptor kinase 5 elevates blood pressure, which segregates with sex and is dependent on Gi-mediated signaling. Circulation. 112:1145-1153.

-- “cAMP accumulation was measured by use of a Bridge-It cAMP assay kit according to the manufacturer’s instructions (Mediomics, LLC).”

2. Calebiro D, de Filippis T, Lucchi S, Covino C, Panigone S, Beck-Peccoz P, Dunlap D, Persani L. (2005) Intracellular entrapment of wild-type TSH receptor by oligomerization with mutants linked to dominant TSH resistance. Hum Mol Genet.14:2991-3002.

-- “cAMP assay was performed using Bridge-It cAMP Designer Fluorescence Assay (Mediomics LLC) in 96-well, black, round-bottom plates, according to manufacturer's instructions.”

1. Bernier SG, Lazarus DD, Clark E, Doyle B, Labenski MT, Thompson CD, Westlin WF, Hannig G. (2004) A methionine aminopeptidase-2 inhibitor, PPI-2458, for the treatment of rheumatoid arthritis. Proc Natl Acad Sci U S A. 101:10768-10773.

-- “For protein detection in cell lysates, we used MetAP-2 polyclonal antibody CM33 (Zymed), proliferating cell nuclear antigen (PCNA) monoclonal antibody PC-10 (Santa Cruz Biotechnology), and MetAP-1 polyclonal antibody (Mediomics, St. Louis).”

If you would like to add a publication that uses one of our product, please contact us and we would be happy to include it.