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Customer Publications

  1. Reina-Campos M, Linares JF, Duran A, Cordes T, L'Hermitte A, Badur MG, Bhangoo MS, Thorson PK, Richards A, Rooslid T, Garcia-Olmo DC, Nam-Cha SY, Salinas-Sanchez AS, Eng K, Beltran H, Scott DA, Metallo CM, Moscat J, Diaz-Meco MT. Increased Serine and One-Carbon Pathway Metabolism by PKCλ/ι Deficiency Promotes Neuroendocrine Prostate Cancer. Cancer Cell. 2019 Feb 28.
    • Quantification of intracellular SAM levels was performed according to manufacturer’s instructions (Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay, 96-well microplate format, Mediomics).
  2. Mayorga L, Salassa BN, Marzese DM, Loos MA, Eiroa HD, Lubieniecki F, García Samartino C, Romano PS, Roqué M. Mitochondrial stress triggers a pro-survival response through epigenetic modifications of nuclear DNA. Cell Mol Life Sci. 2019 Jan 23.
    • Fluorometric measurement of S‑adenosyl‑methionine (HSkM cells) Bridge-It® S-adenosyl methionine (SAM) fluorescence assay kit (Mediomics®) was used according to the manufacturer's instructions.
  3. Agrawal Singh S, Lerdrup M, Gomes AR, van de Werken HJ, Vilstrup Johansen J, Andersson R, Sandelin A, Helin K, Hansen K. PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells. Elife. 2019 Jan 23;8. pii: e40364.
    • SAM was measured using the Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit from Mediomics according to the manufacturer’s instructions (Mediomics, LLC, St. Louis, Missouri, U.S.A.).
  4. Kitajima S, Ivanova E, Guo S, Yoshida R, Campisi M, Sundararaman SK, Tange S, Mitsuishi Y, Thai TC, Masuda S, Piel BP, Sholl LM, Kirschmeier PT, Paweletz CP, Watanabe H, Yajima M, Barbie DA. Suppression of STING Associated with LKB1 Loss in KRAS-Driven Lung Cancer. Cancer Discov. 2019 Jan;9(1):34-45.
    • The amount of S-adenosylmethionine was measured using the Bridge-IT SAM fluorescence Kit (Mediomics, Cat.# 1-1-10038) according to the manufacturer’s instructions.
  5. Dahl AA, Callewaert DM, inventors; Wellmetris LLC, assignee. Wellness panel. US10161928B2. 2018 Dec 25.
    • Mediomic's L-Tryptophan Assay Kit, available as of Jun. 6, 2006. Web. Accessed on Mar. 25, 2015 (hereinafter referred to as Mediomics'). *
  6. Hsueh CF, Wu HJ, Tsai TS, Wu CL, Chang CK. The Effect of Branched-Chain Amino Acids, Citrulline, and Arginine on High-Intensity Interval Performance in Young Swimmers. Nutrients. 2018 Dec 14;10(12). pii: E1979.
    • Plasma tryptophan concentrations were analyzed with a fluorescence assay (Bridge-It, Mediomics, St. Louis, MO, USA).
  7. Poupeau A, Garde C, Sulek K, Citirikkaya K, Treebak JT, Arumugam M, Simar D, Olofsson LE, Bäckhed F, Barrès R. Genes controlling the activation of natural killer lymphocytes are epigenetically remodeled in intestinal cells from germ-free mice. FASEB J. 2018 Oct 10:fj201800787R.
    • One fraction was used to measure protein concentration by bicinchoninic acid assay, and the other was used to measure the SAM concentration with a fluorescence assay relying on fluorescence resonance energy transfer technology (Bridge-It SAM; Mediomics, St. Louis, MO, USA).
  8. Diana T, Daiber A, Oelze M, Neumann S, Olivo PD, Kanitz M, Stamm P, Kahaly GJ. Stimulatory TSH-Receptor Antibodies and Oxidative Stress in Graves Disease. J Clin Endocrinol Metab. 2018 Oct 1;103(10):3668-3677.
    • Intracellular cAMP levels were measured with a homogeneous, fluorescent assay that is based on binding of cAMP to a DNA-binding protein (Bridge-It® cAMP designer fluorescence assay, Cat. #122934; Mediomics LLC, St. Louis, MO).
  9. Cohen D, Chumakov I, Nabirochkin S, Guedj M, Hajj R, inventors; Pharnext, assignee. Diagnostic tools for alzheimer's disease. US20180275144A1. 2018 Sep 27.
    • L-tryptophan can be measured using Bridge-It® L-Tryptophan Fluorescence Assay (Mediomics) which is based on the activity of tryptophan repressor protein and can detect tryptophan for instance in human urine or serum.
  10. McEachron TA, Triche TJ, Sorenson L, Parham DM, Carpten JD. Profiling targetable immune checkpoints in osteosarcoma. Oncoimmunology. 2018 Sep 11;7(12):e1475873.
    • Tryptophan levels in the conditioned media were assayed using the Bridge-It Tryptophan Fluorescence Assay (Mediomics) according to the manufacturers protocol.
  11. Lane DJR, Bae DH, Siafakas AR, Suryo Rahmanto Y, Al-Akra L, Jansson PJ, Casero RA Jr, Richardson DR. Coupling of the polyamine and iron metabolism pathways in the regulation of proliferation: Mechanistic links to alterations in key polyamine biosynthetic and catabolic enzymes. Biochim Biophys Acta Mol Basis Dis. 2018 Sep;1864(9 Pt B):2793-2813.
    • The determination of AdoMet levels were assessed using the Bridge-It® SAM Fluorescence Assay (Mediomics LLC, St. Louis, MO), based on the manufacturer's instructions.
  12. Li X, Hu J, Easley CJ. Automated microfluidic droplet sampling with integrated, mix-and-read immunoassays to resolve endocrine tissue secretion dynamics. Lab Chip. 2018 Sep 26;18(19):2926-2935.
    • Insulin was quantified using a homogeneous fluorescence assay (Human Insulin FRET-PINCER Assay Kit; Mediomics, St. Louis, MO) within droplets.
  13. Suganuma T, Swanson SK, Gogol M, Garrett TJ, Conkright-Fincham J, Florens L, Washburn MP, Workman JL. MPTAC Determines APP Fragmentation via Sensing Sulfur Amino Acid Catabolism. Cell Rep. 2018 Aug 7;24(6):1585-1596.
    • SAMe Quantification: 8.6–11.1 μg cytoplasmic extracts and 5.7 μg nuclear extracts were subjected to the Bridge-It SAM Fluorescence Assay, following the manufacturer’s instructions (1-1-1004B; Mediomics).
  14. Negou JT, Hu J, Li X, Easley CJ. Advancement of analytical modes in a multichannel, microfluidic droplet-based sample chopper employing phase-locked detection. Anal Methods. 2018 Jul 28;10(28):3436-3443.
    • Concentrations of albumin in human serum samples were quantified using a mix-and-read, homogeneous fluorescence immunoassay (Human Albumin FRET-PINCER Assay Kit; Mediomics, St. Louis, MO)…
  15. Chen X, Schönberger B, Menz J, Ludewig U. Plasticity of DNA methylation and gene expression under zinc deficiency in Arabidopsis roots. Plant Cell Physiol. 2018 May 25.
    • The S-adenosyl methionine level was determined with Bridge-It® S-Adenosyl Methionine Fluorescence Assay Kit (Mediomics, United States), following the manufacturer's instruction.
  16. Heyduk T, Heyduk E, inventors; Saint Louis University, assignee. Methods for the selection of aptamers. US9951376B2. 2018 Apr 24.
    • Patent Citations: ...WO2013016280A2, 2011-07-22, 2013-01-31, Mediomics, Llc, Compositions and methods for selecting aptamers; US20140248710A1, 2005-06-06, 2014-09-04, Mediomics Llc, Three-component biosensors for detecting macromolecules and other analytes; US20160077088A1, 2014-09-12, 2016-03-17, Mediomics Llc, Molecular biosensors with a modular design.
  17. Riopel M, Seo JB, Bandyopadhyay GK, Li P, Wollam J, Chung H, Jung SR, Murphy A, Wilson M, de Jong R, Patel S, Balakrishna D, Bilakovics J, Fanjul A, Plonowski A, Koh DS, Larson CJ, Olefsky JM, Lee YS. Chronic fractalkine administration improves glucose tolerance and pancreatic endocrine function. J Clin Invest. 2018 Apr 2;128(4):1458-1470.
    • After the incubation period, the Bridge-It Cyclic AMP Designer Assay was used to determine intracellular cAMP levels per the manufacturer’s instructions (catalog 122934; Mediomics LLC).
  18. Na H, Ponomarova O, Giese GE, Walhout AJM. C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development. Cell Rep. 2018 Mar 20;22(12):3126-3133.
    • SAM was measured using the Bridge-It S-Adenosyl Methionine Fluorescence Assay Kit purchased from Mediomics following the manufacturer’s recommendations.
  19. Bhadra B, Bhalla R, Kruckeberg AL, Nagarajan V, Patnaik R, Suh W, inventors; Butamax Advanced Biofuels LLC, assignee. Fermentative production of alcohols. US9909148B2. 2018 Mar 6.
    • Alternatively, one could utilize enzyme assay methods to measure phosphodiesterase activity (e.g., Bridge-It® PDE assays, Mediomics, LLC, St. Louis, Mo. …
  20. Samusik N, Nolan GP, Goltsev Y, inventors; The Board of Trustees of the Leland Stanford Junior University, assignee. Biosensor for detecting multiple epitopes on a target. US9909167B2. 2018 Mar 6.
    • Patent Citations: ...US20120028242A1, 2008-11-21, 2012-02-02, Mediomics Llc, Biosensor for detecting multiple epitopes on a target.
  21. Lin YT, Chiu MS, Chang CK. Branched-chain amino acids and arginine improve physical but not skill performance in two consecutive days of exercise. Sci Sport. 2017 Dec; 32(6):e221-e228.
    • Plasma free tryptophan concentration was analyzed with a fluorescence assay according to manufacturer’s recommendation (Bridge-It, Mediomics, St. Louis, MO, USA).
  22. Jung J, Kim LJY, Wang X, Wu Q, Sanvoranart T, Hubert CG, Prager BC, Wallace LC, Jin X, Mack SC, Rich JN. Nicotinamide metabolism regulates glioblastoma stem cell maintenance. JCI Insight. 2017 May 18;2(10). pii: 90019.
    • For measurement of L-methionine, homocysteine, SAM, SAH, and NAD+ conventional assay kits were used for their detection as follows: Bridge-It L-methionine fluorescence assay kit (Mediomics)…
  23. Mahmoud ME, Fereig R, Nishikawa Y. Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice. Infect Immun. 2017 Mar 23;85(4). pii: e00007-17.
    • Plasma L-Trp was assayed with a Bridge-It L-Trp fluorescence assay (Mediomics, St. Louis, MO)…
  24. Zhou Z, Garrow TA, Dong X, Luchini DN, Loor JJ. Hepatic Activity and Transcription of Betaine-Homocysteine Methyltransferase, Methionine Synthase, and Cystathionine Synthase in Periparturient Dairy Cows Are Altered to Different Extents by Supply of Methionine and Choline. J Nutr. 2017 Jan;147(1):11-19.
    • Liver tissue L-Met was measured at −10, 7, 20, and 30 d relative to parturition with the use of a commercial kit (catalog no. TRF-1–1-1006B; Mediomics)…
  25. Portugal L, Muñóz-Garay C, Martínez de Castro DL, Soberón M, Bravo A. Toxicity of Cry1A toxins from Bacillus thuringiensis to CF1 cells does not involve activation of adenylate cyclase/PKA signaling pathway. Insect Biochem Mol Biol. 2017 Jan;80:21-31.
    • The cAMP levels were determined by using the Bridge-It cAMP designer fluorescence assay system (Mediomics, LLC, St. Louis), according to the manufacturer's specifications.
  26. Østergaard O, Follmann F, Olsen AW, Heegaard NH, Andersen P, Rosenkrands I. Quantitative Protein Profiling of Chlamydia trachomatis Growth Forms Reveals Defense Strategies Against Tryptophan Starvation. Mol Cell Proteomics. 2016 Dec;15(12):3540-3550.
    • After sterile filtration, the Trp content in the spent culture medium was determined by the Bridge-It® L-Trp assay according to the manufacturer's instructions (Mediomics, LLC, St. Louis, MO).
  27. Cheng IS, Wang YW, Chen IF, Hsu GS, Hsueh CF, Chang CK. The Supplementation of Branched-Chain Amino Acids, Arginine, and Citrulline Improves Endurance Exercise Performance in Two Consecutive Days. J Sports Sci Med. 2016 Aug 5;15(3):509-515.
    • Plasma tryptophan concentration was analyzed with a fluorescence assay (Bridge-It, Mediomics, St. Louis, MO, USA).
  28. Chen IF, Wu HJ, Chen CY, Chou KM, Chang CK. Branched-chain amino acids, arginine, citrulline alleviate central fatigue after 3 simulated matches in taekwondo athletes: a randomized controlled trial. J Int Soc Sports Nutr. 2016 Jul 13;13:28.
    • Plasma free tryptophan concentration was analyzed with a fluorescence assay (Bridge-It, Mediomics, St. Louis, MO, USA).
  29. Yang CC, Wu CL, Chen IF, Chang CK. Prevention of perceptual-motor decline by branched-chain amino acids, arginine, citrulline after tennis match. Scand J Med Sci Sports. 2017 Sep;27(9):935-944.
    • Plasma total tryptophan concentration was analyzed by a fluorescence assay (Bridge-It, Mediomics, St. Louis, Missouri, USA).
  30. Blain GM, Mangum TS, Sidhu SK, Weavil JC, Hureau TJ, Jessop JE, Bledsoe AD, Richardson RS, Amann M. Group III/IV muscle afferents limit the intramuscular metabolic perturbation during whole body exercise in humans. J Physiol. 2016 Sep 15;594(18):5303-15.
    • Tryptophan levels were analysed using the Bridge‐It L‐Tryptophan Fluorescence Assay (Mediomics, LLC, St Louis, MO, USA).
  31. Kolar K, Wischhusen HM, Müller K, Karlsson M, Weber W, Zurbriggen MD. A synthetic mammalian network to compute population borders based on engineered reciprocal cell-cell communication. BMC Syst Biol. 2015 Dec 30;9:97.
    • Interleukin-4 (IL-4) and L-tryptophan were quantified in the culture medium using an IL-4 ELISA kit (Pepro Tech, cat. no. 900-K14) or the Bridge-IT L-Tryptophan Fluorescence Assay (Mediomics, cat. no. 1-1-1002A) according to the manufacturer’s instructions, respectively.
  32. Mahmoud ME, Ihara F, Fereig RM, Nishimura M, Nishikawa Y. Induction of depression-related behaviors by reactivation of chronic Toxoplasma gondii infection in mice. Behav Brain Res. 2016 Feb 1;298(Pt B):125-33.
    • Plasma l-Trp was measured by fluorescence (excitation: 485 nm, emission: 665 nm) using the Bridge-It l-Trp Fluorescence Assay kit (Mediomics, LLC).
  33. Murray TV, Dong X, Sawyer GJ, Caldwell A, Halket J, Sherwood R, Quaglia A, Dew T, Anilkumar N, Burr S, Mistry RK, Martin D, Schröder K, Brandes RP, Hughes RD, Shah AM, Brewer AC. NADPH oxidase 4 regulates homocysteine metabolism and protects against acetaminophen-induced liver damage in mice. Free Radic Biol Med. 2015 Dec;89:918-30.
    • Methionine was measured in extracts from Wt and Nox4-/- mouse livers using a Bridge-It® l-Methionine (l-Met) Fluorescence Assay (mediomics) as per the manufacturer's protocol.
  34. Masyuk TV, LaRusso NF, Masyuk A, inventors; Mayo Foundation for Medical Education and Research, assignee. Targeting tgr5 to treat disease. US20180236064A1. 2018 Aug 23.
    • Production of cAMP was detected using a Bridge-It cAMP designer cAMP assay (Mediomics, St. Louis, Mo.) according to manufacturer's protocol.
  35. Koczor CA, Jiao Z, Fields E, Russ R, Ludaway T, Lewis W. AZT-induced mitochondrial toxicity: an epigenetic paradigm for dysregulation of gene expression through mitochondrial oxidative stress. Physiol Genomics. 2015 Oct;47(10):447-54.
    • For SAM quantitation, a SAM fluorescence assay was utilized (Mediomics, St. Louis, MO). Cardiac SAM abundance was quantitated against an external standard according to the manufacturer's protocols.
  36. Oh SK, Shin JO, Baek JI, Lee J, Bae JW, Ankamerddy H, Kim MJ, Huh TL, Ryoo ZY, Kim UK, Bok J, Lee KY. Pannexin 3 is required for normal progression of skeletal development in vertebrates. FASEB J. 2015 Nov;29(11):4473-84.
    • After 4 d of micromass culture, the cAMP levels in the cells were measured using the Bridge-It cAMP Designer fluorescence assay kit (Mediomics, St. Louis, MO, USA).
  37. McNelis JC, Lee YS, Mayoral R, van der Kant R, Johnson AM, Wollam J, Olefsky JM. GPR43 Potentiates β-Cell Function in Obesity. Diabetes. 2015 Sep;64(9):3203-17.
    • Intracellular cAMP levels in Min6 cells and isolated islets were measured using Bridge-It cAMP Designer Fluorescence Assay Kit (Mediomics).
  38. Dann SG, Ryskin M, Barsotti AM, Golas J, Shi C, Miranda M, Hosselet C, Lemon L, Lucas J, Karnoub M, Wang F, Myers JS, Garza SJ, Follettie MT, Geles KG, Klippel A, Rollins RA, Fantin VR. Reciprocal regulation of amino acid import and epigenetic state through Lat1 and EZH2. EMBO J. 2015 Jul 2;34(13):1773-85.
    • Cellular SAM was assayed using the Bridge‐It S‐adenosylmethionine cellular assay kit (Mediomics) as per the manufacturer's instructions.
  39. Röhrig UF, Majjigapu SR, Vogel P, Zoete V, Michielin O. Challenges in the Discovery of Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors. J Med Chem. 2015 Dec 24;58(24):9421-37.
    • 245 000 compounds of Amgen’s proprietary library were screened against IDO1 and IDO2, detecting kynurenine formation by fluorescence and l-Trp consumption by the proprietary Bridge-IT Trp assay (Mediomics, LLC).
  40. Chang CK, Chang Chien KM, Chang JH, Huang MH, Liang YC, Liu TH. Branched-chain amino acids and arginine improve performance in two consecutive days of simulated handball games in male and female athletes: a randomized trial. PLoS One. 2015 Mar 24;10(3):e0121866.
    • Plasma free tryptophan concentration was analyzed with a fluorescence assay according to manufacturer’s recommendation (Bridge-It, Mediomics, St. Louis, MO, USA).
  41. Nishikawa K, Iwamoto Y, Kobayashi Y, Katsuoka F, Kawaguchi S, Tsujita T, Nakamura T, Kato S, Yamamoto M, Takayanagi H, Ishii M. DNA methyltransferase 3a regulates osteoclast differentiation by coupling to an S-adenosylmethionine-producing metabolic pathway. Nat Med. 2015 Mar;21(3):281-7.
    • ATP and SAM in osteoclast precursors and osteoclasts were detected using the Kinshiro ATP Luminescence kit (CosmoBio) and Bridge-It SAM Fluorescence Assay Kit (Mediomics), respectively.
  42. Piesiewicz A, Kedzierska U, Turkowska E, Adamska I, Majewski PM. Seasonal postembryonic maturation of the diurnal rhythm of serotonin in the chicken pineal gland. Chronobiol Int. 2015 Feb;32(1):59-70.
    • Pineal glands were sonicated (Bandelin electronic, Berlin, Germany) in 25 ml of ice-cold sodium phosphate buffer (50mM, pH 6.8) and duplicate 10 ml aliquots were taken to measure TRP concentration with a Bridge-It L-tryptophan Fluorescence Assay Kit (Mediomics, St. Louis, MO) using a microplate fluorometer (Ascent, Thermo-labsystems, Milford, MA; excitation 485 nm/emission 680 nm).
  43. Mews P, Zee BM, Liu S, Donahue G, Garcia BA, Berger SL. Histone methylation has dynamics distinct from those of histone acetylation in cell cycle reentry from quiescence. Mol Cell Biol. 2014 Nov;34(21):3968-80.
    • To quantify SAM levels, the Bridge-It SAM fluorescence assay (Mediomics) was used according to the manufacturer’s instructions.
  44. Urata Y, Koga K, Hirota Y, Akiyama I, Izumi G, Takamura M, Nagai M, Harada M, Hirata T, Yoshino O, Kawana K, Fujii T, Osuga Y. IL-1β increases expression of tryptophan 2,3-dioxygenase and stimulates tryptophan catabolism in endometrioma stromal cells. Am J Reprod Immunol. 2014 Nov;72(5):496-503.
    • Concentration of tryptophan in conditioned media was measured using Bridge-It L-Tryptophan Fluorescence Assay (Mediomics, St. Louis, MO, USA) according to the manufacturer's protocol.
  45. Salvatori AS, Elrick MM, Samson WK, Corbett JA, Yosten GL. Neuronostatin inhibits glucose-stimulated insulin secretion via direct action on the pancreatic α-cell. Am J Physiol Endocrinol Metab. 2014 Jun 1;306(11):E1257-63.
    • Sample pellets were resuspended in appropriate assay buffers, and cAMP was measured by radioimmunoassay (PerkinElmer), BioTrak enzyme immunoassay (Amersham, Piscataway, NJ), or Bridge-It fluorometric assay (Mediomics, St. Louis, MO) according to the manufacturers' protocols.
  46. Tan W, Thomas P. Activation of the Pi3k/Akt pathway and modulation of phosphodiesterase activity via membrane progestin receptor-alpha (mPRalpha) regulate progestin-initiated sperm hypermotility in Atlantic croaker. Biol Reprod. 2014 May;90(5):105.
    • Phosphodiesterase activity was measured using the Bridge-It cAMP PDE Assay kit (Mediomics, St. Louis, MO).
  47. Saalau-Bethell SM, Berdini V, Cleasby A, Congreve M, Coyle JE, Lock V, Murray CW, O'Brien MA, Rich SJ, Sambrook T, Vinkovic M, Yon JR, Jhoti H. Crystal structure of human soluble adenylate cyclase reveals a distinct, highly flexible allosteric bicarbonate binding pocket. ChemMedChem. 2014 Apr;9(4):823-32.
    • The enzymatic activity of hsolAC was measured in 96-well (black) plate format using the Mediomics Bridge-It Designer cAMP kit (cat #122 935, Mediomics, LLC, St. Louis, MO, USA).
  48. Batra V, Verma P. Dietary L-methionine supplementation mitigates gamma-radiation induced global DNA hypomethylation: enhanced metabolic flux towards S-adenosyl-L-methionine (SAM) biosynthesis increases genomic methylation potential. Food Chem Toxicol. 2014 Jul;69:46-54.
    • l-methionine and SAM assay kit was purchased from Mediomics Inc. (St. Louis, MO, USA).
  49. Mandal S, Mukhopadhyay S, Bandhopadhyay S, Sen G, Biswas T. 14-Deoxyandrographolide alleviates ethanol-induced hepatosteatosis through stimulation of AMP-activated protein kinase activity in rats. Alcohol. 2014 Mar;48(2):123-32.
    • Intracellular cAMP levels in rat hepatocytes and human hepatoma HepG2 cells (105 /mL) were measured by using Bridge-It ® cAMP Fluorescence Assay kits (Mediomics, LLC).
  50. Zhu H, Guariglia S, Li W, Brancho D, Wang ZV, Scherer PE, Chow CW. Role of extracellular signal-regulated kinase 5 in adipocyte signaling. J Biol Chem. 2014 Feb 28;289(9):6311-22.
    • Phosphodiesterase activity was determined by using the Bridge-It cAMP-PDE assay kits from Mediomics.
  51. Ishikawa M, Iwamoto T, Fukumoto S, Yamada Y. Pannexin 3 inhibits proliferation of osteoprogenitor cells by regulating Wnt and p21 signaling. J Biol Chem. 2014 Jan 31;289(5):2839-51.
    • The level of cAMP was determined with a Bridge-It cAMP designer fluorescence assay kit (Mediomics) and measured as previously described.
  52. Lo TF, Tsai WC, Chen ST. MicroRNA-21-3p, a berberine-induced miRNA, directly down-regulates human methionine adenosyltransferases 2A and 2B and inhibits hepatoma cell growth. PLoS One. 2013 Sep 30;8(9):e75628.
    • SAM levels in the supernatants were quantified using the Bridge-It SAM fluorescence assay kit (Mediomics) and detected using SpectraMax plate reader (Molecular Devices) according to the manufacturer’s instructions.
  53. Garciandia A, Suarez T. The NMRA/NMRAL1 homologue PadA modulates the expression of extracellular cAMP relay genes during aggregation in Dictyostelium discoideum. Dev Biol. 2013 Sep 15;381(2):411-22.
    • Phosphodiesterase activity was measured in freshly obtained conditioned media, from 1×107 cells/mL starved for 6.5 h (plated at 2×106 cells/cm2), using Bridge-It cAMP_PDE Assay (Mediomics LLC, IL, USA).
  54. Masyuk AI, Huang BQ, Radtke BN, Gajdos GB, Splinter PL, Masyuk TV, Gradilone SA, LaRusso NF. Ciliary subcellular localization of TGR5 determines the cholangiocyte functional response to bile acid signaling. Am J Physiol Gastrointest Liver Physiol. 2013 Jun 1;304(11):G1013-24.
    • cAMP levels in cholangiocytes were measured by the Bridge-It cAMP designer cAMP assay (Mediomics, St. Louis, MO), as we have previously described, and expressed in picomoles of cAMP per microgram of protein (H69 cells) or in picomoles of cAMP per microgram of DNA (microdissected rat IBDs).
  55. Lee YS, Morinaga H, Kim JJ, Lagakos W, Taylor S, Keshwani M, Perkins G, Dong H, Kayali AG, Sweet IR, Olefsky J. The fractalkine/CX3CR1 system regulates β cell function and insulin secretion. Cell. 2013 Apr 11;153(2):413-25.
    • Intracellular cAMP level of Min6 cells was measured using Bridge-it cAMP assay kit (Mediomics, LLC, USA).
  56. Stollar V, Mensah V, Adams S, Li ML. Evolution of Sindbis virus with a low-methionine-resistant phenotype is dependent both on a pre-existing mutation and on the methionine concentration in the medium. PLoS One. 2013;8(3):e60504.
    • The concentration of AdoMet in cells was determined by the Bridge-It S-Adenosyl Methionine Fluorescence Assay following the manufacturer’s protocol (Mediomics).
  57. Schrötter A, Pfeiffer K, El Magraoui F, Platta HW, Erdmann R, Meyer HE, Egensperger R, Marcus K, Müller T. The amyloid precursor protein (APP) family members are key players in S-adenosylmethionine formation by MAT2A and modify BACE1 and PSEN1 gene expression-relevance for Alzheimer's disease. Mol Cell Proteomics. 2012 Nov;11(11):1274-88.
    • S-adenosylmethionine (SAM) levels were measured with Bridge-It™ S-adenosylmethionine Fluorescence Assay (Mediomics, St. Louis, MO, USA) using a 384-well microplate format.
  58. Piesiewicz A, Kedzierska U, Adamska I, Usarek M, Zeman M, Skwarlo-Sonta K, Majewski PM. Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken. Gen Comp Endocrinol. 2012 Nov 1;179(2):143-51.
    • Pineal glands were sonicated (Bandelin electronic) in 25 μl of ice-cold sodium phosphate buffer (50 mM, pH 6.8) and duplicate 10 μl aliquots were taken to measure TRP concentration using a Bridge-It l-tryptophan Fluorescence Assay Kit (Mediomics).
  59. Kanellopoulos AK, Semelidou O, Kotini AG, Anezaki M, Skoulakis EM. Learning and memory deficits consequent to reduction of the fragile X mental retardation protein result from metabotropic glutamate receptor-mediated inhibition of cAMP signaling in Drosophila. J Neurosci. 2012 Sep 19;32(38):13111-24.
    • The Bridge-It cAMP designer fluorescence assay system (Mediomics) was used to measure cAMP levels in all samples.
  60. Bacchus W, Lang M, El-Baba MD, Weber W, Stelling J, Fussenegger M. Synthetic two-way communication between mammalian cells. Nat Biotechnol. 2012 Oct;30(10):991-6.
    • L-tryptophan levels in the medium and serum were profiled using a fluorescence-based assay kit (Mediomics, St. Louis, MO, USA, cat. no. 1-1-1001B).
  61. Baldwin AC, Green CD, Olson LK, Moxley MA, Corbett JA. A role for aberrant protein palmitoylation in FFA-induced ER stress and β-cell death. Am J Physiol Endocrinol Metab. 2012 Jun 1;302(11):E1390-8.
    • Insulin accumulating in the incubation buffer was measured using the Human Insulin Pincer assay kit (Mediomics, St. Louis, MO).
  62. Mlinaric-Rascan I, Milek M, Smid A, Kuzelicki NK. S-Adenosylmethionine: A Novel Factor in the Individualization of Thiopurine Therapy In: Sanoudou D, editor. Clinical Applications of Pharmacogenetics. Rijeka: IntechOpen; 2012.
    • The commercially available assay for SAM determination is a Mediomics Bridge-It® fluorescence assay based on a combination of fluorescence measurement techniques…
  63. Nan YS, Li SY, Kang JL, Suzuki S, Ema Y, Nishiwaki K. Different effects of dibutyryl cAMP on monolayer permeability in human aortic and coronary arterial endothelial cells. Afr J Microbiol Res. 2012 Feb 9;6(5):897-903.
    • Measurement of intracellular cAMP in endothelial cells Intracellular cAMP was measured using a Bridge-It cAMP designer fluorescence assay kit (Mediomics, St. Louis, MO, USA) according to the manufacturer's instructions.
  64. Meininger D, Zalameda L, Liu Y, Stepan LP, Borges L, McCarter JD, Sutherland CL. Purification and kinetic characterization of human indoleamine 2,3-dioxygenases 1 and 2 (IDO1 and IDO2) and discovery of selective IDO1 inhibitors. Biochim Biophys Acta. 2011 Dec;1814(12):1947-54.
    • The activities and inhibition of IDO1, IDO2 and TDO were determined by measuring the production of the kynurenine product by kynurenine fluorescence or by the consumption of the Trp substrate using the Bridge-IT tryptophan fluorescence assay (Mediomics, St. Louis, MO).
  65. Abukhashim M, Wiebe GJ, Seubert JM. Regulation of forskolin-induced cAMP production by cytochrome P450 epoxygenase metabolites of arachidonic acid in HEK293 cells. Cell Biol Toxicol. 2011 Oct;27(5):321-32.
    • Intracellular cAMP levels were measured by using commercially available kits (Mediomics).
  66. McGraw DW, Brown KM. A Polymorphic Change in Amino Acid 220 (Ser220Cys) Of The Human β2- Adrenergic Receptor Encodes Receptors With Different Gs-Coupled Signal Transduction Properties A65. Mechanisms of Airway Response to Injury (Respiratory Viruses, Cigarette Smoke, and Asthma). 2011 May 1; A2072-A2072.
    • Cellular production of 125 cAMP was measured using a fluorescent assay kit (Mediomics, MO).
  67. Kazutaka S, Winnall WR, Muir JA, Hedger MP. Regulation of Sertoli cell activin A and inhibin B by tumour necrosis factor α and interleukin 1α: interaction with follicle-stimulating hormone/adenosine 3',5'-cyclic phosphate signalling. Mol Cell Endocrinol. 2011 Mar 30;335(2):195-203.
    • The commercially available assay for SAM determination is a Mediomics Bridge-It® fluorescence assay based on a combination of fluorescence measurement techniques.
  68. Reszka KJ, Sallans L, Macha S, Brown K, McGraw DW, Kovacic MB, Britigan BE. Airway peroxidases catalyze nitration of the β2-agonist salbutamol and decrease its pharmacological activity. J Pharmacol Exp Ther. 2011 Feb;336(2):440-9.
    • The ability of native and nitrated salbutamol to stimulate cAMP production in airway smooth muscle cells was measured by a fluorescent detection assay using a commercially available kit (Mediomics, St. Louis, MO).
  69. Piesiewicz A, Podobas E, Kędzierska U, Joachimiak E, Markowska M, Majewski P, Skwarlo-Sonta K. Season-Related Differences in the Biosynthetic Activity of the Neonatal Chicken Pineal Gland Open Ornithol J. 2010 Oct 13;3(1):134-140.
    • L-tryptophan was measured in duplicate pineal sonicate samples using a Bridge-It L-Tryptophan Fluorescence Assay Kit (Mediomics), according to the manufacturer’s protocol
  70. Nifoussi SK. Posttranslational regulation of protein function and stability at the mitochondria and beyond. PhD (Doctor of Philosophy) thesis, University of Iowa, 2010.
    • Bridge-It cAMP designer assay (Mediomics) was used based on manufacturer's protocol.
  71. Iwamoto T, Nakamura T, Doyle A, Ishikawa M, de Vega S, Fukumoto S, Yamada Y. Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation. J Biol Chem. 2010 Jun 11;285(24):18948-58.
    • The level of cAMP was determined with a Bridge-It cAMP Designer fluorescence assay kit (Mediomics).
  72. Batra V, Sridhar S, Devasagayam TP. Enhanced one-carbon flux towards DNA methylation: Effect of dietary methyl supplements against gamma-radiation-induced epigenetic modifications. Chem Biol Interact. 2010 Feb 12;183(3):425-33.
    • SAM assay kit was purchased from Mediomics Inc. (St. Louis, MO, USA).
  73. Steffens A. Low-dose of sodium arsenite causes delayed differentiation in C2C12 mouse myoblast cells through the repression of the transcription factor myogenin. Master's thesis, Clemson University, 2009.
    • S-adenosyl methionine (SAM) concentrations were determined using the Bridge-It SAM Fluorescence Assay (Mediomics, St. Louis, MO).
  74. Alvarado JJ, Nemkal A, Sauder JM, Russell M, Akiyoshi DE, Shi W, Almo SC, Weiss LM. Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470. Mol Biochem Parasitol. 2009 Dec;168(2):158-67.
    • Recombinant human MetAP2 was purchased from Mediomics (St Louis, MO).
  75. Watson JA, Watson CJ, McCrohan AM, Woodfine K, Tosetto M, McDaid J, Gallagher E, Betts D, Baugh J, O'Sullivan J, Murrell A, Watson RW, McCann A. Generation of an epigenetic signature by chronic hypoxia in prostate cells. Hum Mol Genet. 2009 Oct 1;18(19):3594-604.
    • SAM levels were quantified using the Bridge-It® SAM fluorescence assay (Mediomics).
  76. Akullian LC, Stevenson CA, Yurkovetskiy AV, Benson JD, Fram RJ, Lowinger TB. Anti-angiogenic and Anti-tumor Activity of XMT-1107, a Fumagillol-derived Polymer Conjugate, and its in vivo Release Product XMT-1191. AACR Annual Meeting. 2009 May;69(9)Suppl:670.
    • MetAP2 inhibition assays were performed at Mediomics, St. Louis, MO. Special thanks to Yie-Hwa Chang.
  77. Masyuk AI, Gradilone SA, Banales JM, Huang BQ, Masyuk TV, Lee SO, Splinter PL, Stroope AJ, Larusso NF. Cholangiocyte primary cilia are chemosensory organelles that detect biliary nucleotides via P2Y12 purinergic receptors. Am J Physiol Gastrointest Liver Physiol. 2008 Oct;295(4):G725-34.
    • After perfusion, an IBD was removed from the microperfusion system, and cAMP levels were measured by using the Bridge-It cAMP designer cAMP assay (Mediomics, St. Louis, MO).
  78. Furukawa F, Kanehara S, Harano F, Shinohara S, Kamimura J, Kawabata S, Igarashi S, Kawamura M, Yamamoto Y, Miyachi Y. Effects of adenosine 5'-monophosphate on epidermal turnover. Arch Dermatol Res. 2008 Oct;300(9):485-93.
    • Intracellular cAMP level was measured with Bridge-It cAMP designer fluorescence assay kit (Mediomics, LLC, St. Louis, MO, USA).
  79. Raake PW, Vinge LE, Gao E, Boucher M, Rengo G, Chen X, DeGeorge BR Jr, Matkovich S, Houser SR, Most P, Eckhart AD, Dorn GW 2nd, Koch WJ. G protein-coupled receptor kinase 2 ablation in cardiac myocytes before or after myocardial infarction prevents heart failure. Circ Res. 2008 Aug 15;103(4):413-22.
    • Isolated adult cardiac myocytes from sham GRK2 fl/fl and αMHC-Cre x GRK2 fl/fl were plated and after 24 hours, cAMP levels were measured using a fluorescence assay (Mediomics, LLC, St. Louis, Missouri, USA) according to the manufacturer’s instructions.
  80. Coppin JF, Qu W, Waalkes MP. Interplay between cellular methyl metabolism and adaptive efflux during oncogenic transformation from chronic arsenic exposure in human cells. J Biol Chem. 2008 Jul 11;283(28):19342-50.
    • SAM levels were assayed according to manufacturer instructions using the Bridge-it® SAM fluorescence assay kit (Mediomics, St. Louis, MO).
  81. Chan A, Tchantchou F, Graves V, Rozen R, Shea TB. Dietary and genetic compromise in folate availability reduces acetylcholine, cognitive performance and increases aggression: critical role of S-adenosyl methionine. J Nutr Health Aging. 2008 Apr;12(4):252-61.
    • SAM levels in homogenates of forebrain were determined by comparison to SAM standards using a Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit (Mediomics, LLC).
  82. Kelley DJ, Davidson RJ, Elliott JL, Lahvis GP, Yin JC, Bhattacharyya A. The cyclic AMP cascade is altered in the fragile X nervous system. PLoS One. 2007 Sep 26;2(9):e931.
    • The Bridge-It cAMP designer fluorescence assay system (Mediomics, LLC, St. Louis, MO) was used to measure cyclic AMP levels in all samples.
  83. Masyuk TV, Masyuk AI, Torres VE, Harris PC, Larusso NF. Octreotide inhibits hepatic cystogenesis in a rodent model of polycystic liver disease by reducing cholangiocyte adenosine 3',5'-cyclic monophosphate. Gastroenterology. 2007 Mar;132(3):1104-16.
    • cAMP concentrations were determined by the Bridge-It cAMP designer cAMP assay (Mediomics, LLC, St Louis, MO).
  84. Chan A, Shea TB. Supplementation with apple juice attenuates presenilin-1 overexpression during dietary and genetically-induced oxidative stress. J Alzheimers Dis. 2006 Dec;10(4):353-8.
    • SAM levels in homogenates of forebrain and in AJC were determined by comparison to SAM standards using a Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit (Mediomics, LLC) assayed on a Hitachi F2000 fluorescent spectrophotometer.
  85. Masyuk AI, Masyuk TV, Splinter PL, Huang BQ, Stroope AJ, LaRusso NF. Cholangiocyte cilia detect changes in luminal fluid flow and transmit them into intracellular Ca2+ and cAMP signaling. Gastroenterology. 2006 Sep;131(3):911-20.
    • The concentrations of cAMP were determined by the Bridge-It cAMP designer cAMP assay (Mediomics, LLC, St. Louis, MO).
  86. Wilson BD, Ii M, Park KW, Suli A, Sorensen LK, Larrieu-Lahargue F, Urness LD, Suh W, Asai J, Kock GA, Thorne T, Silver M, Thomas KR, Chien CB, Losordo DW, Li DY. Netrins promote developmental and therapeutic angiogenesis. Science. 2006 Aug 4;313(5787):640-4.
    • Two different cAMP measurement systems were utilized (Mediomics and Assay Designs); similar results were obtained with each assessment.
  87. Zhang X, Candas M, Griko NB, Taussig R, Bulla LA Jr. A mechanism of cell death involving an adenylyl cyclase/PKA signaling pathway is induced by the Cry1Ab toxin of Bacillus thuringiensis. Proc Natl Acad Sci USA. 2006 Jun 27;103(26):9897-902
    • Cells were incubated with isobutyl-1-methylxanthine (0.5 mM) before the addition of Cry1Ab, and cAMP was measured by using the Bridge-It cAMP Assay (Mediomics) in S5 and H5 cells(3 x 104).
  88. Brennan JP, Bardswell SC, Burgoyne JR, Fuller W, Schröder E, Wait R, Begum S, Kentish JC, Eaton P. Oxidant-induced activation of type I protein kinase A is mediated by RI subunit interprotein disulfide bond formation. J Biol Chem. 2006 Aug 4;281(31):21827-36.
    • cAMP was measured in cells treated with H2O2 or isoprenaline using a Bridge-It cAMP designer fluorescence assay kit according to the manufacturer's guidelines (Mediomics).
  89. Hannig G, Lazarus DD, Bernier SG, Karp RM, Lorusso J, Qiu D, Labenski MT, Wakefield JD, Thompson CD, Westlin WF. Inhibition of melanoma tumor growth by a pharmacological inhibitor of MetAP-2, PPI-2458. Int J Oncol. 2006 Apr;28(4):955-63.
    • For protein detection in cell lysates, we used the following antibodies: MetAP-1 polyclonal antibody (Mediomics)...
  90. Cooper AC, Karp RM, Clark EJ, Taghizadeh NR, Hoyt JG, Labenski MT, Murray MJ, Hannig G, Westlin WF, Thompson CD. A novel methionine aminopeptidase-2 inhibitor, PPI-2458, inhibits non-Hodgkin's lymphoma cell proliferation in vitro and in vivo. Clin Cancer Res. 2006 Apr 15;12(8):2583-90.
    • Human recombinant MetAP-2 (Mediomics, St. Louis, MO), prebound to the biotinylated PPI-2458 analogue, was used to generate a standard curve for quantitation for each individual assay.
  91. White CA, Sowa JR, Blake AD. A fluorescence-based second messenger assay to identify small molecule antagonists of somatostatin. ASPET. 2006 Mar;48(1):31.
    • Intracellular cAMP levels were quantified using a Bridge-It cAMP Designer Fluorescence Assay (Mediomics, LLC, St Louis, MO), which replaced more conventional radioimmunoassay methods.
  92. Kalhan R, Thavarajah K, Nlend MC, Nair A, Smith LJ, Sporn PHS. The soy isoflavone genistein blocks transforming growth factor b1‐stimulated lung fibroblast to myofibroblast transformation. J Investig Med. 2006;54:S354.
    • Using an assay in which reductions in fluorescence intensity are associated with increasing cAMP levels (Mediomics, LLC), we quantified cAMP levels in neurospheres (NS) and differentiated cells (DC) from the human cortical fragile X (M049) and control…
  93. Calebiro D, de Filippis T, Lucchi S, Covino C, Panigone S, Beck-Peccoz P, Dunlap D, Persani L. Intracellular entrapment of wild-type TSH receptor by oligomerization with mutants linked to dominant TSH resistance. Hum Mol Genet. 2005 Oct 15;14(20):2991-3002.
    • cAMP assay was performed using Bridge-It cAMP Designer Fluorescence Assay (Mediomics LLC) in 96-well, black, round-bottom plates, according to manufacturer's instructions.
  94. Keys JR, Zhou RH, Harris DM, Druckman CA, Eckhart AD. Vascular smooth muscle overexpression of G protein-coupled receptor kinase 5 elevates blood pressure, which segregates with sex and is dependent on Gi-mediated signaling. Circulation. 2005 Aug 23;112(8):1145-53.
    • cAMP accumulation was measured by use of a Bridge-It cAMP assay kit according to the manufacturer’s instructions (Mediomics, LLC).
  95. Bernier SG, Lazarus DD, Clark E, Doyle B, Labenski MT, Thompson CD, Westlin WF, Hannig G. A methionine aminopeptidase-2 inhibitor, PPI-2458, for the treatment of rheumatoid arthritis. Proc Natl Acad Sci USA. 2004 Jul 20;101(29):10768-73.
    • Human recombinant MetAP-2 (Mediomics), prebound to the biotinylated PPI-2458 analog, was used to generate the standard curve.

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