Showing all 5 results
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Bridge-It® Cyclic AMP (cAMP) all in one Assay Kit, 384-well format
Bridge-It® Cyclic AMP (cAMP) all in one Assay Kit, 384-well format
The Bridge-It® cAMP assay is highly specific assay to quantify cAMP in stimulated cells. ATP, AMP, and cGMP have all been tested for selectivity using the cAMP assay. No response was detected using the Bridge-It® cAMP assay with any of these compounds within the concentration range that is expected in biological samples (i.e., millimolar range for AMP and ATP, and micromolar range for cGMP).
An all-in-one solution is added to activated cells and incubated for 30 minutes. Clarified lysate samples are transferred to a black 384-well microplate to read the fluorescence signal.
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Bridge-It® L-Tryptophan Fluorescence Assay Kit for Plasma and Serum Samples, 384-well format
Bridge-It® L-Tryptophan Fluorescence Assay Kit for Plasma and Serum Samples, 384-well format
The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.
The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.
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TR-FRET Bridge-It® L-Tryptophan Fluorescence Assay Kit for Plasma and Serum Samples, 384-well format
TR-FRET Bridge-It® L-Tryptophan Fluorescence Assay Kit for Plasma and Serum Samples, 384-well format
The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.
The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.
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TR-FRET Bridge-It® L-Tryptophan Fluorescence Assay Kit, 384-well format
TR-FRET Bridge-It® L-Tryptophan Fluorescence Assay Kit, 384-well format
The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.
The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.
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TR-FRET Bridge-It® L-Tryptophan Fluorescence Assay Kit, 96-well format
TR-FRET Bridge-It® L-Tryptophan Fluorescence Assay Kit, 96-well format
The Bridge-It® tryptophan fluorescence assay is based on the activity of tryptophan repressor protein (TrpR), a bacterial DNA-binding protein. TrpR protein binds to its DNA-binding site in tryptophan-dependent fashion.
The central feature of this assay design is the TrpR-dependent association of two fluorochrome-labeled DNA half-fragments (one labeled with fluorescein and the other labeled with Oyster® 645 fluophore). Each fragment contains about one-half of the TrpR protein DNA-binding site. In the presence of L-tryptophan an increase in fluorescence signal can be detected as a result of the tryptophan-dependent association of the labeled DNA half-fragments. Tryptophan is readily detectable using the Bridge-It® tryptophan fluorescence assay in various types of test samples including bacterial growth medium, brain extract, yeast extract, as well as in human serum and urine.
