Recombinant human methionine aminopeptidase-2

Recombinant human methionine aminopeptidase-2 purified from Sf21 cells.

Catalog No: PM-803262-100 µg

SOURCE:

The recombinant human methionine aminopeptidase-2 was expressed in Sf21 cells and purified according the procedures described in BBRC 227:152-157, 1996. Protein Concentration was determined by using a Bio-Rad protein assay kit. The purity of the protein was determined by SDS-PAGE.

PRODUCT:

Each vial contains ~ 100 μg purified human methionine aminopeptidase-2 in 20 mM Hepes, pH 7.3, 0.1M KCl, 10% glycerol, 1 mM Co(II), 1.5 mM MgCl2 and 0.02% NaN3

STORAGE:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Store at -70ºC.

PURITY:

Single band by SDS-PAGE under reducing condition visualized by Coomassie Blue Staining.

Customer Publications using Mediomics’ recombinant human MetAP2:

1. Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470. Alvarado JJ, Nemkal A, Sauder JM, Russell M, Akiyoshi DE, Shi W, Almo SC, Weiss LM. Mol Biochem Parasitol. 2009 Dec;168(2):158-67. doi: 10.1016/j.molbiopara.2009.07.008.

2. Inhibition of melanoma tumor growth by a pharmacological inhibitor of MetAP-2, PPI-2458. Hannig G, Lazarus DD, Bernier SG, Karp RM, Lorusso J, Qiu D, Labenski MT, Wakefield JD, Thompson CD, Westlin WF. Int J Oncol. 2006 Apr;28(4):955-63.

3. A novel methionine aminopeptidase-2 inhibitor, PPI-2458, inhibits non-Hodgkin’s lymphoma cell proliferation in vitro and in vivo. Cooper AC, Karp RM, Clark EJ, Taghizadeh NR, Hoyt JG, Labenski MT, Murray MJ, Hannig G, Westlin WF, Thompson CD. Clin Cancer Res. 2006 Apr 15;12(8):2583-90.

4. A methionine aminopeptidase-2 inhibitor, PPI-2458, for the treatment of rheumatoid arthritis. Bernier SG, Lazarus DD, Clark E, Doyle B, Labenski MT, Thompson CD, Westlin WF, Hannig G. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10768-73.

RESEARCH USE:

For research use only, not for use in developing information for submission to the FDA in support of an application for approval of a drug or medical device. Any commercial use of these products, including for the development of commercial products, requires an additional license from Mediomics, LLC.

This product  is protected by U.S. Patents 5,888,796 and 5,885,820.

Product info :

Recombinant human methionine aminopeptidase-2 Product info

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