Mouse IgG FRET-PINCER® Assay Kit, 384-well format
Mediomics’ mouse immunoglobulin G (mIgG) assay kit can be used to quantitatively detect the concentration of mIgG in biological samples. Product applications include drug and vaccine testing, detection of mIgG antibodies, and determining mIgG concentration. This kit is ideally suited for the rapid, simultaneous measurement of mouse IgG levels in large number of test samples due to its speed and simplicity. In comparison to the ELISA procedure, the TRF-PINCER® technology provides a simple (one mixing step) and fast (30 minute incubation) assay that is quantitative and reproducible.
$150.00 – $450.00
Description
This assay was developed for use in a 384-well plate format. A 96-well format is also available. Please inquire within.
Mediomics’ mouse immunoglobulin G (mIgG) FRET-PINCER® assay kit can be used to quantitatively detect the concentration of mIgG in biological samples.
Immunoglobulin G (IgG) is the most abundant immunoglobulin in serum, and it is a biomarker of the adaptive immune response. Immunoglobulin levels can vary with health status, age, and drug treatment; therefore, quantitative measurements are a useful tool for pre-clinical researchers. Overabundance or, conversely, an absence of a certain specific subclass may be associated with disease.
Product applications:
1. Drug and vaccine testing to identify the presence/absence of an adaptive immune response.
2. Rapid detection of IgG antibodies in a pull-down or co-IP assay.
3. Detection of mIgG concentration within biological samples as part of a pre-clinical research program.
Product Advantage:
Mediomics’ mIgG FRET-PINCER® assay is ideally suited for the rapid, simultaneous measurement of mouse IgG levels in large number of test samples due to its speed and simplicity. It is readily adaptable to the high-throughput screening platforms currently being used in drug discovery research.
In comparison to the ELISA procedure, the FRET-PINCER® technology provides a simple (one mixing step) and fast (30 minute incubation) assay that is quantitative and reproducible.
Fig. 1: Mouse IgG PINCER® Standard curve
Fig. 2: Linear range of the Mouse IgG PINCER® Standard curve
Note: These standard curves are for reference only. Please generate your own standard curve.
Limit of Detection (LOD): 3.9 ng/mL
CV value: < 7%
Detection Range: 3.9 – 1000 ng/mL
Pricing: The 100-well kit is a one-time trial purchase. For bulk pricing options, please contact us. For any other questions or comments, please do not hesitate to contact us. We are always happy to help!
Please refer to our technology pages for information on the scientific principles and technological advancements of the FRET- PINCER® assays.
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PINCER® is a registered trademark of Mediomics, LLC, St. Louis, Missouri, U.S.A. Mediomics has a worldwide, exclusive license for this assay platform from Saint Louis University, St. Louis, Missouri.
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The PINCER® Assay kit is provided for laboratory R&D use only. This product is not approved by the U.S. Government or by the government of any other country of the world for use in the clinical diagnosis of disease, or treatment of disease in humans or animals.
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Patents allowed and pending in the U.S. and abroad.
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PINCER® products have been developed with support, in part, from the National Institutes of Health, through STTR Phase I and Phase II Grants.
Catalog #: MIG1012-100 , MIG1012-384
Protocol:
MSDS: