Biotin TRF-PINCER Assay Kit, 384-well format


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This assay was developed for use in a 384-well plate format. A 96-well format is also available. Please inquire within.


This Homogeneous TRF-PINCER® Assay is intended for quick screening and quantification of Biotin in biological samples.

Biotin, also known as vitamin H or B7, is a water-soluble B-complex vitamin which plays an indispensable role in numerous naturally occurring carboxylation reactions in living cells. Biotin is also very commonly used to label peptides, DNA or proteins. The strong binding between biotin and the tetrameric protein avidin, streptavidin, or neutravidin (with a dissociation constant (Kd) in the order of 10-14 M) allow biotin to be used in many different biotechnological applications. The Biotin PINCER® Assay Kit provides a convenient method to quantify protein concentration in unknown solution as well as estimate the amount of biotin in biotinylated samples.


Product Applications:

  1. Quickly detect and quantify the concentration of streptavidin-labeled primary antibodies within biological samples.

  2. Trace streptavidin-labeled proteins and particles during fluorescent imaging experiments.

  3. Amplify the signal of low-abundance targets by combining the streptavidin-SA interaction with stable double-strand DNA interactions to optimize your assay.

  4. Customize the intensity of the fluorescence signal (see below).


Principle of the Assay

Two biotinylated PINCERS have oligonucleotides (with short complementary sequences) labeled with fluorescent probes. The PINCERS will form a complex upon binding to streptavidin and activate Time-Resolved Fluorescence FRET (fluorescence resonance energy transfer).

The fluorescence signals can be customized to fit within an appropriate measurable range by adding non-fluorescently labeled PINCERS. When non-fluorescently labeled biotinylated PINCERS are present, they will compete with the fluorescence-labeled biotinylated PINCER and result in a decreased TRF-FRET signal (see figure below). Please inquire for more details.

 biotin concept



The protocol for the TRF-PINCER® Assay is simple and fast.


h Insulin Assay Pic


biotin trf std curve

Limit of Detection (LOD): 7.8 nM

CV: <3.5%


Product Features:

  • Easy
    Mix test sample or standard with the assay solution and incubate at ~25°C

  • Fast
    Read fluorescent signal after 30 minutes of incubation

  • Sensitive
    Assay measures biotin with an LOD of 7.8 nM.

  • Flexible
    Assay is adaptable to both low- and high-throughput screening formats

Pricing: The 100-well kit is a one-time trial purchase. For bulk pricing options, please contact us. For any other questions or comments, please do not hesitate to contact us. We are always happy to help!


Please refer to our technology pages for information on the scientific principles and technological advancements of the TRF-PINCER® assays.


  1. PINCER® is a registered trademark of Mediomics, LLC, St. Louis, Missouri, U.S.A. Mediomics has a worldwide, exclusive license for this assay platform from Saint Louis University, St. Louis, Missouri.

  2. The TRF-PINCER® Assay kit is provided for laboratory R&D use only. This product is not approved by the U.S. Government or by the government of any other country of the world for use in the clinical diagnosis of disease, or treatment of disease in humans or animals.

  3. Patents allowed and pending in the U.S. and abroad.

  4. PINCER® products have been developed with support, in part, from the National Institutes of Health, through STTR Phase I and Phase II Grants.

Catalog #:  BTTR-1006-100 , BTTR-1006-384