Mouse IgM PINCER® Assay Kit, 384-well format
Mediomics’ mouse immunoglobulin M (mIgM) TRF-PINCER® assay is ideal for quantitatively detecting the concentration of mIgM in biological samples. Product applications include monitoring IgM antibody production, detection of mIgM antibodies, and determining mIgM concentration. This kit is ideally suited for the rapid, simultaneous measurement of mouse IgM levels in large number of test samples due to its speed and simplicity. In comparison to the ELISA procedure, the TRF-PINCER® technology provides a simple (one mixing step) and fast (30 minute incubation) assay that is quantitative and reproducible.
$150.00 – $450.00
Description
This assay was developed for use in a 384-well plate format. A 96-well format is also available. Please inquire within.
Mediomics’ mouse immunoglobulin M (mIgM) FRET-PINCER® assay kit can be used to quantitatively detect the concentration of mIgM in biological samples.
Immunoglobulin M (IgM) is a class of antibodies found in circulating body fluids. The IgM heavy chain is usually a pentamer constructed from five basic monomer units which are covalently linked together with disulfide bonds. The presence of J chains also stabilizes the pentameric molecules. It has high molecular weight of 970,000 Daltons and 10 antigen binding sites. IgM can also exist as hexamer while the J chain is not presented in this form. IgM is often the first antibody isotype to be secreted during an adaptive immune response. IgM cannot cross the placental barrier, thus it is a promising isotype to use in the design of monoclonal antibody biologics.
Product applications:
1. Monitoring production of IgM class antibodies.
2. Rapid detection of IgM antibodies in a pull-down or co-IP assay.
3. Detection of mIgM concentration within biological samples as part of a pre-clinical research program.
Product Advantage:
Mediomics’ mIgM FRET-PINCER® assay is ideally suited for the rapid, simultaneous measurement of mouse IgM levels in large number of test samples due to its speed and simplicity. It is readily adaptable to the high-throughput screening platforms currently being used in drug discovery research.
In comparison to the ELISA procedure, the FRET-PINCER® technology provides a simple (one mixing step) and fast (30 minute incubation) assay that is quantitative and reproducible.
Note: The standard curve is for reference only. Please generate your own standard curve.
Limit of Detection (LOD): 60 ng/mL
CV value: < 5%
Detection Range: 60 – 8000 ng/mL
Specificity: No cross reaction observed with human, rabbit, and goat IgG. There is some cross reaction (< 10%) observed with mouse IgG and the extent is depended on different IgG subtypes.
Pricing: The 100-well kit is a one-time trial purchase. For bulk pricing options, please contact us. For any other questions or comments, please do not hesitate to contact us. We are always happy to help!
Please refer to our technology pages for information on the scientific principles and technological advancements of the TRF-PINCER® assays.
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PINCER® is a registered trademark of Mediomics, LLC, St. Louis, Missouri, U.S.A. Mediomics has a worldwide, exclusive license for this assay platform from Saint Louis University, St. Louis, Missouri.
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The PINCER® Assay kit is provided for laboratory R&D use only. This product is not approved by the U.S. Government or by the government of any other country of the world for use in the clinical diagnosis of disease, or treatment of disease in humans or animals.
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Patents allowed and pending in the U.S. and abroad.
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PINCER® products have been developed with support, in part, from the National Institutes of Health, through STTR Phase I and Phase II Grants.
