“Quantification of intracellular SAM levels was performed according to manufacturer’s instructions (Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay, 96-well microplate format, Mediomics).”
“Fluorometric measurement of S‑adenosyl‑methionine (HSkM cells) Bridge-It® S-adenosyl methionine (SAM) fluorescence assay kit (Mediomics®) was used according to the manufacturer’s instructions.”
“SAM was measured using the Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit from Mediomics according to the manufacturer’s instructions (Mediomics, LLC, St. Louis, Missouri, U.S.A.).”
5. Suppression of STING Associated with LKB1 Loss in KRAS-Driven Lung Cancer. Kitajima S, Ivanova E, Guo S, Yoshida R, Campisi M, Sundararaman SK, Tange S, Mitsuishi Y, Thai TC, Masuda S, Piel BP, Sholl LM, Kirschmeier PT, Paweletz CP, Watanabe H, Yajima M, Barbie DA. Cancer Discov. 2019 Jan;9(1):34-45.
“The amount of S-adenosylmethionine was measured using the Bridge-IT SAM fluorescence Kit (Mediomics, Cat.# 1-1-10038) according to the manufacturer’s instructions.”
“Mediomic’s L-Tryptophan Assay Kit, available as of Jun. 6, 2006. Web. <http://search.cosmobio.co.jp/cosmo_search_p/search_gate2/docs/MDM_/111001B.20060615.pdf> Accessed on Mar. 25, 2015 (hereinafter referred to as Mediomics’). *”
“One fraction was used to measure protein concentration by bicinchoninic acid assay, and the other was used to measure the SAM concentration with a fluorescence assay relying on fluorescence resonance energy transfer technology (Bridge-It SAM; Mediomics, St. Louis, MO, USA).”
“Intracellular cAMP levels were measured with a homogeneous, fluorescent assay that is based on binding of cAMP to a DNA-binding protein (Bridge-It® cAMP designer fluorescence assay, Cat. #122934; Mediomics LLC, St. Louis, MO).”
“L-tryptophan can be measured using Bridge-It® L-Tryptophan Fluorescence Assay (Mediomics) which is based on the activity of tryptophan repressor protein and can detect tryptophan for instance in human urine or serum.”
“Tryptophan levels in the conditioned media were assayed using the Bridge-It Tryptophan Fluorescence Assay (Mediomics) according to the manufacturers protocol.”
“The determination of AdoMet levels were assessed using the Bridge-It® SAM Fluorescence Assay (Mediomics LLC, St. Louis, MO), based on the manufacturer’s instructions.”
“SAMe Quantification: 8.6–11.1 μg cytoplasmic extracts and 5.7 μg nuclear extracts were subjected to the Bridge-It SAM Fluorescence Assay, following the manufacturer’s instructions (1-1-1004B; Mediomics).”
“Concentrations of albumin in human serum samples were quantified using a mix-and-read, homogeneous fluorescence immunoassay (Human Albumin FRET-PINCER Assay Kit; Mediomics, St. Louis, MO)…”
“The S-adenosyl methionine level was determined with Bridge-It® S-Adenosyl Methionine Fluorescence Assay Kit (Mediomics, United States), following the manufacturer’s instruction.”
“After the incubation period, the Bridge-It Cyclic AMP Designer Assay was used to determine intracellular cAMP levels per the manufacturer’s instructions (catalog 122934; Mediomics LLC).”
“SAM was measured using the Bridge-It S-Adenosyl Methionine Fluorescence Assay Kit purchased from Mediomics following the manufacturer’s recommendations.”
20. Fermentative production of alcohols. Bhadra B, Bhalla R, Kruckeberg AL, Nagarajan V, Patnaik R, Suh W, inventors; Butamax Advanced Biofuels LLC, assignee. US9909148B2. 2018 Mar 6.
“Alternatively, one could utilize enzyme assay methods to measure phosphodiesterase activity (e.g., Bridge-It® PDE assays, Mediomics, LLC, St. Louis, Mo. …”
“Plasma free tryptophan concentration was analyzed with a fluorescence assay according to manufacturer’s recommendation (Bridge-It, Mediomics, St. Louis, MO, USA).”
“For measurement of L-methionine, homocysteine, SAM, SAH, and NAD+ conventional assay kits were used for their detection as follows: Bridge-It L-methionine fluorescence assay kit (Mediomics)…”
“Liver tissue L-Met was measured at −10, 7, 20, and 30 d relative to parturition with the use of a commercial kit (catalog no. TRF-1–1-1006B; Mediomics)…”
“The cAMP levels were determined by using the Bridge-It cAMP designer fluorescence assay system (Mediomics, LLC, St. Louis), according to the manufacturer’s specifications.”
“After sterile filtration, the Trp content in the spent culture medium was determined by the Bridge-It® L-Trp assay according to the manufacturer’s instructions (Mediomics, LLC, St. Louis, MO).”
“Interleukin-4 (IL-4) and L-tryptophan were quantified in the culture medium using an IL-4 ELISA kit (Pepro Tech, cat. no. 900-K14) or the Bridge-IT L-Tryptophan Fluorescence Assay (Mediomics, cat. no. 1-1-1002A) according to the manufacturer’s instructions, respectively.”
“Methionine was measured in extracts from Wt and Nox4-/- mouse livers using a Bridge-It® l-Methionine (l-Met) Fluorescence Assay (mediomics) as per the manufacturer’s protocol.”
35. Targeting tgr5 to treat disease. Masyuk TV, LaRusso NF, Masyuk A, inventors; Mayo Foundation for Medical Education and Research, assignee. US20180236064A1. 2018 Aug 23.
“Production of cAMP was detected using a Bridge-It cAMP designer cAMP assay (Mediomics, St. Louis, Mo.) according to manufacturer’s protocol.”
“For SAM quantitation, a SAM fluorescence assay was utilized (Mediomics, St. Louis, MO). Cardiac SAM abundance was quantitated against an external standard according to the manufacturer’s protocols.”
“After 4 d of micromass culture, the cAMP levels in the cells were measured using the Bridge-It cAMP Designer fluorescence assay kit (Mediomics, St. Louis, MO, USA).”
“245 000 compounds of Amgen’s proprietary library were screened against IDO1 and IDO2, detecting kynurenine formation by fluorescence and l-Trp consumption by the proprietary Bridge-IT Trp assay (Mediomics, LLC).”
“Plasma free tryptophan concentration was analyzed with a fluorescence assay according to manufacturer’s recommendation (Bridge-It, Mediomics, St. Louis, MO, USA).”
“ATP and SAM in osteoclast precursors and osteoclasts were detected using the Kinshiro ATP Luminescence kit (CosmoBio) and Bridge-It SAM Fluorescence Assay Kit (Mediomics), respectively.”
“Pineal glands were sonicated (Bandelin electronic, Berlin, Germany) in 25 ml of ice-cold sodium phosphate buffer (50mM, pH 6.8) and duplicate 10 ml aliquots were taken to measure TRP concentration with a Bridge-It L-tryptophan Fluorescence Assay Kit (Mediomics, St. Louis, MO) using a microplate fluorometer (Ascent, Thermo-labsystems, Milford, MA; excitation 485 nm/emission 680 nm).”
“Concentration of tryptophan in conditioned media was measured using Bridge-It L-Tryptophan Fluorescence Assay (Mediomics, St. Louis, MO, USA) according to the manufacturer’s protocol.”
“Sample pellets were resuspended in appropriate assay buffers, and cAMP was measured by radioimmunoassay (PerkinElmer), BioTrak enzyme immunoassay (Amersham, Piscataway, NJ), or Bridge-It fluorometric assay (Mediomics, St. Louis, MO) according to the manufacturers’ protocols.”
“The enzymatic activity of hsolAC was measured in 96-well (black) plate format using the Mediomics Bridge-It Designer cAMP kit (cat #122 935, Mediomics, LLC, St. Louis, MO, USA).”
“Intracellular cAMP levels in rat hepatocytes and human hepatoma HepG2 cells (105 /mL) were measured by using Bridge-It ® cAMP Fluorescence Assay kits (Mediomics, LLC).”
“SAM levels in the supernatants were quantified using the Bridge-It SAM fluorescence assay kit (Mediomics) and detected using SpectraMax plate reader (Molecular Devices) according to the manufacturer’s instructions.”
“Phosphodiesterase activity was measured in freshly obtained conditioned media, from 1×107 cells/mL starved for 6.5 h (plated at 2×106 cells/cm2), using Bridge-It cAMP_PDE Assay (Mediomics LLC, IL, USA).”
“cAMP levels in cholangiocytes were measured by the Bridge-It cAMP designer cAMP assay (Mediomics, St. Louis, MO), as we have previously described, and expressed in picomoles of cAMP per microgram of protein (H69 cells) or in picomoles of cAMP per microgram of DNA (microdissected rat IBDs).”
“The concentration of AdoMet in cells was determined by the Bridge-It S-Adenosyl Methionine Fluorescence Assay following the manufacturer’s protocol (Mediomics).”
“S-adenosylmethionine (SAM) levels were measured with Bridge-It™ S-adenosylmethionine Fluorescence Assay (Mediomics, St. Louis, MO, USA) using a 384-well microplate format.”
“Pineal glands were sonicated (Bandelin electronic) in 25 μl of ice-cold sodium phosphate buffer (50 mM, pH 6.8) and duplicate 10 μl aliquots were taken to measure TRP concentration using a Bridge-It l-tryptophan Fluorescence Assay Kit (Mediomics).”
“The commercially available assay for SAM determination is a Mediomics Bridge-It® fluorescence assay based on a combination of fluorescence measurement techniques…”
“Measurement of intracellular cAMP in endothelial cells Intracellular cAMP was measured using a Bridge-It cAMP designer fluorescence assay kit (Mediomics, St. Louis, MO, USA) according to the manufacturer’s instructions.”
“The activities and inhibition of IDO1, IDO2 and TDO were determined by measuring the production of the kynurenine product by kynurenine fluorescence or by the consumption of the Trp substrate using the Bridge-IT tryptophan fluorescence assay (Mediomics, St. Louis, MO).”
“The commercially available assay for SAM determination is a Mediomics Bridge-It® fluorescence assay based on a combination of fluorescence measurement techniques.”
“The ability of native and nitrated salbutamol to stimulate cAMP production in airway smooth muscle cells was measured by a fluorescent detection assay using a commercially available kit (Mediomics, St. Louis, MO).”
“L-tryptophan was measured in duplicate pineal sonicate samples using a Bridge-It L-Tryptophan Fluorescence Assay Kit (Mediomics), according to the manufacturer’s protocol”
“After perfusion, an IBD was removed from the microperfusion system, and cAMP levels were measured by using the Bridge-It cAMP designer cAMP assay (Mediomics, St. Louis, MO).”
“Isolated adult cardiac myocytes from sham GRK2 fl/fl and αMHC-Cre x GRK2 fl/fl were plated and after 24 hours, cAMP levels were measured using a fluorescence assay (Mediomics, LLC, St. Louis, Missouri, USA) according to the manufacturer’s instructions.”
“SAM levels in homogenates of forebrain were determined by comparison to SAM standards using a Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit (Mediomics, LLC).”
“SAM levels in homogenates of forebrain and in AJC were determined by comparison to SAM standards using a Bridge-It S-Adenosyl Methionine (SAM) Fluorescence Assay Kit (Mediomics, LLC) assayed on a Hitachi F2000 fluorescent spectrophotometer.”
“The concentrations of cAMP were determined by the Bridge-It™ cAMP designer cAMP assay (Mediomics, LLC, St. Louis, MO). ”
87. Netrins promote developmental and therapeutic angiogenesis. Wilson BD, Ii M, Park KW, Suli A, Sorensen LK, Larrieu-Lahargue F, Urness LD, Suh W, Asai J, Kock GA, Thorne T, Silver M, Thomas KR, Chien CB, Losordo DW, Li DY. Science. 2006 Aug 4;313(5787):640-4.
“Two different cAMP measurement systems were utilized (Mediomics and Assay Designs); similar results were obtained with each assessment.”
“ICells were incubated with isobutyl-1-methylxanthine (0.5 mM) before the addition of Cry1Ab, and cAMP was measured by using the Bridge-It cAMP Assay (Mediomics) in S5 and H5 cells(3 x 104). ”
“cAMP was measured in cells treated with H2O2 or isoprenaline using a Bridge-It cAMP designer fluorescence assay kit according to the manufacturer’s guidelines (Mediomics).”
“Human recombinant MetAP-2 (Mediomics, St. Louis, MO), prebound to the biotinylated PPI-2458 analogue, was used to generate a standard curve for quantitation for each individual assay.”
“Intracellular cAMP levels were quantified using a Bridge-It cAMP Designer Fluorescence Assay (Mediomics, LLC, St Louis, MO), which replaced more conventional radioimmunoassay methods.”
“Using an assay in which reductions in fluorescence intensity are associated with increasing cAMP levels (Mediomics, LLC), we quantified cAMP levels in neurospheres (NS) and differentiated cells (DC) from the human cortical fragile X (M049) and control…”
“cAMP assay was performed using Bridge-It cAMP Designer Fluorescence Assay (Mediomics LLC) in 96-well, black, round-bottom plates, according to manufacturer’s instructions.”
