Showing all 2 results

• Bridge-It® cAMP-Phosphodiesterase (PDE) Assay Kit, 384-well format

  QUICKVIEW
  Select options

  Bridge-It® cAMP-Phosphodiesterase (PDE) Assay Kit, 384-well format

  The Bridge-It® PDE Assay is a fluorescence-based, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase activity from a purified source and determining the IC 50 of their inhibitors. This assay is based on the format of Bridge-It® cAMP all in one Fluorescence Assay (Mediomics, Cat# 122938).

  Adenosine-3’,5’-cyclic monophosphate (cAMP) is an important second messenger which is involved in the modulation of numerous biological processes. The measurement of cAMP is especially important in new drug discovery since cAMP levels are closely related to the activity of one of the major targets for new drug discovery – the G protein-coupled receptors (GPCR).

  Select options

• Bridge-It® Cyclic AMP (cAMP) Designer Assay Kit, 96-well format

  QUICKVIEW
  Select options

  Bridge-It® Cyclic AMP (cAMP) Designer Assay Kit, 96-well format

  The Bridge-It® cAMP assay is a highly specific assay to quantify cAMP in stimulated cells. ATP, AMP, and cGMP have all been tested for selectivity using the cAMP assay. No response was detected using the Bridge-It® cAMP assay with any of these compounds within the concentration range that is expected in biological samples (i.e., millimolar range for AMP and ATP, and micromolar range for cGMP).

  An all-in-one solution is added to activated cells and incubated for 30 minutes.  Clarified lysate samples are transferred to a black 96-well microplate to read the fluorescence signal.

  Select options